Identification of four small molecular mass proteins in the silk of Bombyx mori

被引:80
作者
Nirmala, X
Mita, K
Vanisree, V
Zurovec, M
Sehnal, F
机构
[1] Acad Sci Czech Republ, Inst Entomol, CR-37005 Ceske Budejovice, Czech Republic
[2] Univ S Bohemia, Fac Biol Sci, Ceske Budejovice, Czech Republic
[3] Natl Inst Radiol Sci, Genome Res Grp, Inage Ku, Chiba 260, Japan
关键词
silk; seroin; proteinase inhibitor; protease inhibitor; antimicrobial agents; Lepidoptera;
D O I
10.1046/j.0962-1075.2001.00282.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This paper describes cDNAs of four small-size proteins that occur in the cocoon silk of Bombyx mori. Two of them (9.9 and 10.3 kDa), which have the N-terminal sequences and the spacing of a few amino acids at C-termini similar to the seroin of Galleria mellonella, are called seroin 1 and seroin 2. The corresponding genes are expressed in the middle, and to a small extent also in the posterior silk gland sections. The seroin I and less conspicuously the seroin 2 mRNAs accumulate in the course of the last larval instar to a maximum in postspinning larvae. Two other proteins (6 kDa and 4.7 kDa) of B. mori cocoons were identified as a typical Kunitz-type and a somewhat unusual Kazal-type proteinase inhibitors, and named BmSPI 1 and BmSPI 2, respectively. Their genes are expressed in the middle, and the BmSPI 1 gene slightly also in the posterior silk gland sections. The expression ensues a few days after the last larval ecdysis and increases until the cocoon spinning. Post-spinning larvae still contain high amounts of the BmSPI 1 but no BmSPI 2 transcripts. It is assumed that seroins and proteinase inhibitors are involved in cocoon protection against predators and microbes.
引用
收藏
页码:437 / 445
页数:9
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