Adsorption properties of a gold-binding peptide assessed by its attachment to a recombinant apoferritin molecule

被引:13
作者
Ishikawa, Kazutaka [1 ]
Yamada, Kiyohito [1 ,2 ]
Kumagai, Shinya [2 ]
Sano, Ken-Ichi [3 ,4 ]
Shiba, Kiyotaka [3 ,4 ]
Yamashita, Ichiro [1 ,2 ,4 ]
Kobayashi, Mime [1 ,4 ]
机构
[1] Nara Inst Sci & Technol, Grad Sch Mat Sci, Nara 6300192, Japan
[2] Matsushita Elect Ind Co Ltd, Adv Technol Res Labs, Kyoto 6190237, Japan
[3] Japanese Fdn Canc Res, Inst Canc, Koto Ku, Dept Prot Engn, Tokyo 1358550, Japan
[4] Japan Sci & Technol Agcy, Core Res Evolut Sci & Technol, Kawaguchi, Saitama 3320012, Japan
关键词
D O I
10.1143/APEX.1.034006
中图分类号
O59 [应用物理学];
学科分类号
摘要
The adsorption properties of a recombinant apoferritin protein fused to a gold-binding peptide were characterized. The results of quartz crystal microbalance measurements showed that the fusion protein preferentially adsorbs to gold surfaces. Scanning electron microscopy also revealed that the protein selectively adsorbed onto a nanometer-scale gold pattern on a SiO2 surface fabricated by electron-beam lithography. Our results indicate that nanodots and nanowires synthesized using a biotemplate can be selectively placed onto a gold surface by genetically modifying the outer surface of the biotemplate. This technique represents an important step toward biotemplate-mediated fabrication of a nanometer-scaled device that utilizes gold electrodes. (C) 2008 The Japan Society of Applied Physics.
引用
收藏
页码:0340061 / 0340063
页数:3
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