Glial Cell Line-derived Neurotrophic Factor-overexpressing Human Neural Stem/Progenitor Cells Enhance Therapeutic Efficiency in Rat with Traumatic Spinal Cord Injury

被引：23

作者：

Hwang, Kyujin ^{[1
,2
]}

Jung, Kwangsoo ^{[3
]}

Kim, Il-Sun ^{[2
]}

Kim, Miri ^{[2
]}

Han, Jungho ^{[3
]}

Lim, Joohee ^{[3
]}

Shin, Jeong Eun ^{[3
]}

Jang, Jae-Hyung ^{[4
]}

Park, Kook In ^{[1
,2
,3
]}

机构：

[1] Yonsei Univ, Coll Med, Brain Korea 21 Plus Project Med Sci, Seoul 03722, South Korea

[2] Yonsei Univ, Coll Med, Yonsei Biomed Res Inst, Seoul 03722, South Korea

[3] Yonsei Univ, Coll Med, Severance Childrens Hosp, Dept Pediat, Seoul 03722, South Korea

[4] Yonsei Univ, Dept Chem & Biomol Engn, Seoul 03722, South Korea

来源：

EXPERIMENTAL NEUROBIOLOGY | 2019年 / 28卷 / 06期

基金：

新加坡国家研究基金会;

关键词：

Spinal cord injuries; Glial cell line-derived neurotrophic factor; Neural stem/progenitor cells; Cell-based therapy; Mechanical allodynia; Paraplegia; PROPRIOSPINAL AXONAL REGENERATION; PROMOTES FUNCTIONAL RECOVERY; STEM-CELLS; NEUROPATHIC PAIN; PROGENITOR-CELLS; INTRASPINAL TRANSPLANTATION; SCHWANN-CELLS; GENE-TRANSFER; GDNF; ALLODYNIA;

D O I：

10.5607/en.2019.28.6.679

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Spinal cord injury (SCI) causes axonal damage and demyelination, neural cell death, and comprehensive tissue loss, resulting in devastating neurological dysfunction. Neural stem/progenitor cell (NSPCs) transplantation provides therapeutic benefits for neural repair in SCI, and glial cell linederived neurotrophic factor (GDNF) has been uncovered to have capability of stimulating axonal regeneration and remyelination after SCI. In this study, to evaluate whether GDNF would augment therapeutic effects of NSPCs for SCI, GDNF-encoding or mock adenoviral vector-transduced human NSPCs (GDNF-or Mock-hNSPCs) were transplanted into the injured thoracic spinal cords of rats at 7 days after SCI. Grafted GDNF-hNSPCs showed robust engraftment, long-term survival, an extensive distribution, and increased differentiation into neurons and oligodendroglial cells. Compared with Mock-hNSPC- and vehicle-injected groups, transplantation of GDNF-hNSPCs significantly reduced lesion volume and glial scar formation, promoted neurite outgrowth, axonal regeneration and myelination, increased Schwann cell migration that contributed to the myelin repair, and improved locomotor recovery. In addition, tract tracing demonstrated that transplantation of GDNF-hNSPCs reduced significantly axonal dieback of the dorsal corticospinal tract (dCST), and increased the levels of dCST collaterals, propriospinal neurons (PSNs), and contacts between dCST collaterals and PSNs in the cervical enlargement over that of the controls. Finally grafted GDNF-hNSPCs substantially reversed the increased expression of voltage-gated sodium channels and neuropeptide Y, and elevated expression of GABA in the injured spinal cord, which are involved in the attenuation of neuropathic pain after SCI. These findings suggest that implantation of GDNF-hNSPCs enhances therapeutic efficiency of hNSPCs-based cell therapy for SCI.

引用

页码：679 / 696

页数：18

共 71 条

[1] The GDNF family: Signalling, biological functions and therapeutic value [J].