Snake extract-laden hemostatic bioadhesive gel cross-linked by visible light

被引：145

作者：

Guo, Yicheng ^{[1
,2
]}

Wang, Ying ^{[1
]}

Zhao, Xiaohong ^{[1
]}

Li, Xue ^{[1
]}

Wang, Quan ^{[3
]}

Zhong, Wen ^{[4
]}

Mequanint, Kibret ^{[5
,6
]}

Zhan, Rixing ^{[1
]}

Xing, Malcolm ^{[2
]}

Luo, Gaoxing ^{[1
]}

机构：

[1] Third Mil Med Univ, Army Med Univ, Southwest Hosp, Inst Burn Res,State Key Lab Trauma Burn & Combine, Chongqing 400038, Peoples R China

[2] Univ Manitoba, Dept Mech Engn, Winnipeg, MB R3T 2N2, Canada

[3] Shantou Univ, Dept Civil Engn, Shantou 515063, Peoples R China

[4] Univ Manitoba, Dept Biosyst Engn, Winnipeg, MB R3T 2N2, Canada

[5] Univ Western Ontario, Dept Chem & Biochem Engn, London, ON N6A 5B9, Canada

[6] Univ Western Ontario, Sch Biomed Engn, London, ON N6A 5B9, Canada

来源：

SCIENCE ADVANCES | 2021年 / 7卷 / 29期

基金：

中国国家自然科学基金; 加拿大自然科学与工程研究理事会;

关键词：

BOTHROPS-ATROX; ADHESIVES; HYDROGEL; VENOM; IDENTIFICATION; PURIFICATION; CHEMISTRY; SYSTEMS;

D O I：

10.1126/sciadv.abf9635

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bioadhesives reduce operation time and surgical complications. However, in the presence of blood, adhesion strength is often compromised. Inspired by the blood clotting activity of snake venom, we report a visible light-induced blood-resistant hemostatic adhesive (HAD) containing gelatin methacryloyl and reptilase, which is a hemocoagulase (HC) extracted from Bothrops atrox. HAD leads to the activation and aggregation of platelets and efficiently transforms fibrinogen into fibrin to achieve rapid hemostasis and seal the tissue. Blood clotting time with HAD was about 45 s compared with 5 to 6 min without HAD. HAD instantaneously achieved hemostasis on liver incision (similar to 45 s) and cut rat tail (similar to 34 s) and reduced blood loss by 79 and 78%, respectively. HAD is also efficient in sealing severely injured liver and abdominal aorta. HAD has great potential to bridge injured tissues by combing hemostasis with adhesives.

引用

页数：15

共 60 条

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