Synthesis of hybrid transition-metalloproteins via thiol-selective covalent anchoring of Rh-phosphine and Ru-phenanthroline complexes

被引：16

作者：

den Heeten, Rene ^{[2
]}

Munoz, Bianca K. ^{[1
]}

Popa, Gina ^{[1
]}

Laan, Wouter ^{[1
]}

Kamer, Paul C. J. ^{[1
]}

机构：

[1] Univ St Andrews, Dept Chem, EaStCHEM, St Andrews KY16 9ST, Fife, Scotland

[2] Univ Amsterdam, Vant Hoff Inst Mol Sci, NL-1018 WV Amsterdam, Netherlands

来源：

DALTON TRANSACTIONS | 2010年 / 39卷 / 36期

关键词：

PHOTOACTIVE YELLOW PROTEIN; BIOTIN-AVIDIN TECHNOLOGY; P-COUMARIC ACID; HALOPHILIC PHOTOTROPHIC BACTERIUM; ARTIFICIAL METALLOENZYMES; ASYMMETRIC HYDROGENATION; ENANTIOSELECTIVE CATALYSIS; DIRECTED EVOLUTION; ACTIVE-SITE; HYDROFORMYLATION;

D O I：

10.1039/c0dt00239a

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

The preparation of hybrid transition metalloproteins by thiol-selective incorporation of organometallic rhodium- and ruthenium complexes is described. Phosphine ligands and two rhodium-diphosphine complexes bearing a carboxylic acid group were coupled to the cysteine of PYP R52G, yielding a metalloenzyme active in the rhodium catalyzed hydrogenation of dimethyl itaconate. The successful coupling was shown by P-31 NMR spectroscopy and ESI mass spectroscopy. In addition wild-type PYP (PYP WT), PYP R52G and ALBP were successfully modified with a (eta(6)-arene) ruthenium(II) phenanthroline complex via a maleimide linker.

引用

页码：8477 / 8483

页数：7

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