Redox potential is a determinant in the Escherichia coli anaerobic fermentative growth and survival:: effects of impermeable oxidant

Decrease of redox potential (E-h) down to -550-600 mV in the Escherichia coli culture is observed during growth in either anaerobic or aerobic conditions. The E. coli growth and survival under anaerobic fermentative conditions were found to be strongly inhibited by potassium ferricyanide in the concentration of 1 mM, when E-h was decreased to - 50-100 mV. This oxidant also resulted in approximately 2-fold decrease of total and N, N'-dicyclohexylcarbodiimide (DCCD)-inhibited H+ efflux, 2.5-fold inhibition in K+ influx, 1.5-fold less K+ accumulation, and delayed a decrease in E-h to negative values by bacteria. K-3[Fe(CN)(6)] was shown to block an ATP-dependent increase in the amount of accessible thiol groups of membrane vesicles that was inhibited by DCCD, and this inhibition by the oxidant could be recovered by dithiothreitol. These effects were not observed with cells growing under aerobic conditions. The effects of K-3[Fe(CN)(6)], an impermeable oxidant, might be explained by the fact that redox potential is a determinant in the E. coli anaerobic fermentative growth and survival that has a regulatory role in maintaining H+ and K fluxes and the number of accessible thiol groups on membrane. (C) 2000 Elsevier Science S.A. All rights reserved.