Is Alba an RNase P subunit?

被引：7

作者：

Ellis, J. Chris ^{[2
]}

Barnes, Jeffrey ^{[3
]}

Brown, James W. ^{[1
]}

机构：

[1] N Carolina State Univ, Dept Microbiol, Raleigh, NC 27695 USA

[2] NIEHS, Lab Signal Transduct, Res Triangle Pk, NC 27709 USA

[3] Appl Biosyst Inc, Foster City, CA USA

来源：

RNA BIOLOGY | 2007年 / 4卷 / 03期

基金：

美国国家科学基金会;

关键词：

ribonuclease P; archaea; rihozyme; Methanothermobacter thermoautotrophicus; Mth1483; Rpp25;

D O I：

10.4161/rna.4.3.5347

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

It has been suggested that Alba, a well-established chromatin protein in Archaea, is also a subunit of the archaeal RNase P holoenzyme, based on the observation that the homolog of this protein in humans has been shown to be associated with RNase P activity. Using the same biochemical methods we used previously to show that four other proteins homologous to eukaryotic RNase P proteins are bona fide RNase P subunits in Archaea, we could not detect any association of the Alba homolog in Methanothermobacter thermoautotrophicus (Mth 1483p) with the RNase P holoenzyme. In addition, the presence of Mth 1483p did not enhance the activity of RNase P holoenzyme reconstituted from recombinant subunits. In conclusion, we find no evidence that Alba is an RNase P subunit.

引用

页码：169 / 172

页数：4

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