Synthesis of substituted 2H-chromenes catalyzed by lipase immobilized on magnetic multiwalled carbon nanotubes

被引:7
作者
Liu, Jiaxu [1 ]
Zhao, Wenxin [1 ]
Zhang, Liu [1 ]
Zhang, Min [1 ]
Chen, Yanqiang [1 ]
Xu, You [1 ]
Li, Yinghua [2 ]
Wang, Lei [1 ]
机构
[1] Jilin Univ, Sch Life Sci, Key Lab Mol Enzymol & Engn, Minist Educ, Changchun 130023, Peoples R China
[2] First Hosp Jilin Univ, Changchun, Peoples R China
关键词
2H-chromene; enzyme; immobilization; magnetic; multiwalled carbon nanotube; ENZYME IMMOBILIZATION; PROMISCUITY; EFFICIENT; IMPROVEMENT;
D O I
10.1002/bab.1939
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Porcine pancreas lipase (PPL) was immobilized on magnetic multiwalled carbon nanotubes successfully for the synthesis of substituted 2H-chromenes. The catalytic activity of immobilized PPL was much higher than that of free PPL. Effects of reaction medium, temperature, and enzyme dosage were also investigated. Under optimum reaction conditions (acetylacetone (1 mmol), salicylaldehyde (1 equivalent), methanol (10 equivalent), and immobilized PPL (protein content: 30 mg; 65 degrees C; DMF 5 mL; 5 H), the immobilized PPL showed an excellent catalytic performance on the synthesis of substituted 2H-chromenes. Moreover, the immobilized PPL exhibited satisfactory thermostability, operational simplicity, and reusability in this reaction.
引用
收藏
页码:411 / 416
页数:6
相关论文
共 41 条
  • [1] SiO2 coated Fe3O4 magnetic nanoparticle dispersed multiwalled carbon nanotubes based amperometric glucose biosensor
    Baby, Tessy Theres
    Ramaprabhu, S.
    [J]. TALANTA, 2010, 80 (05) : 2016 - 2022
  • [2] Catalytic promiscuity in biocatalysis: Using old enzymes to form new bonds and follow new pathways
    Bornscheuer, UT
    Kazlauskas, RJ
    [J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (45) : 6032 - 6040
  • [3] Hydrolases: catalytically promiscuous enzymes for non-conventional reactions in organic synthesis
    Busto, Eduardo
    Gotor-Fernandez, Vicente
    Gotor, Vicente
    [J]. CHEMICAL SOCIETY REVIEWS, 2010, 39 (11) : 4504 - 4523
  • [4] Two-step desymmetrization of dipyrazolidyl 3-phenylglutarate via lipase-catalyzed hydrolysis in organic solvents
    Chan, Po-Hao
    Tsai, Shau-Wei
    [J]. CHEMICAL ENGINEERING SCIENCE, 2016, 139 : 41 - 48
  • [5] Nanomaterials for biocatalyst immobilization - state of the art and future trends
    Cipolatti, Eliane P.
    Valerio, Alexsandra
    Henriques, Rosana O.
    Moritz, Denise E.
    Ninow, Jorge L.
    Freire, Denise M. G.
    Manoel, Evelin A.
    Fernandez-Lafuente, Roberto
    de Oliveira, Debora
    [J]. RSC ADVANCES, 2016, 6 (106): : 104675 - 104692
  • [6] Biological importance of structurally diversified chromenes
    Costa, Marta
    Dias, Tatiana A.
    Brito, Alexandra
    Proenca, Fernanda
    [J]. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2016, 123 : 487 - 507
  • [7] Chemical oxidation of multiwalled carbon nanotubes
    Datsyuk, V.
    Kalyva, M.
    Papagelis, K.
    Parthenios, J.
    Tasis, D.
    Siokou, A.
    Kallitsis, I.
    Galiotis, C.
    [J]. CARBON, 2008, 46 (06) : 833 - 840
  • [8] Physico-chemical properties, kinetic parameters, and glucose inhibition of several beta-glucosidases for industrial applications
    de Andrades, Diandra
    Graebin, Natalia G.
    Ayub, Marco A. Z.
    Fernandez-Lafuente, Roberto
    Rodrigues, Rafael C.
    [J]. PROCESS BIOCHEMISTRY, 2019, 78 : 82 - 90
  • [9] Efficient lipase-catalyzed Knoevenagel condensation: utilization of biocatalytic promiscuity for synthesis of benzylidene-indolin-2-ones
    Ding, Yan
    Xiang, Xinran
    Gu, Mengjie
    Xu, Haoran
    Huang, He
    Hu, Yi
    [J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING, 2016, 39 (01) : 125 - 131
  • [10] Importance of the Support Properties for Immobilization or Purification of Enzymes
    dos Santos, Jose Cleiton S.
    Barbosa, Oveimar
    Ortiz, Claudia
    Berenguer-Murcia, Angel
    Rodrigues, Rafael C.
    Fernandez-Lafuente, Roberto
    [J]. CHEMCATCHEM, 2015, 7 (16) : 2413 - 2432