A Phenome-Based Functional Analysis of Transcription Factors in the Cereal Head Blight Fungus, Fusarium graminearum

被引：277

作者：

Son, Hokyoung ^{[1
,2
,3
]}

Seo, Young-Su ^{[1
,2
,3
]}

Min, Kyunghun ^{[1
,2
,3
]}

Park, Ae Ran ^{[1
,2
,3
]}

Lee, Jungkwan ^{[4
]}

Jin, Jian-Ming ^{[1
,2
,3
]}

Lin, Yang ^{[1
,2
,3
]}

Cao, Peijian ^{[5
]}

Hong, Sae-Yeon ^{[1
,2
,3
]}

Kim, Eun-Kyung ^{[1
,2
,3
]}

Lee, Seung-Ho ^{[1
,2
,3
]}

Cho, Aram ^{[1
,2
,3
]}

Lee, Seunghoon ^{[1
,2
,3
]}

Kim, Myung-Gu ^{[1
,2
,3
]}

Kim, Yongsoo ^{[1
,2
,3
]}

Kim, Jung-Eun ^{[1
,2
,3
]}

Kim, Jin-Cheol ^{[6
]}

Choi, Gyung Ja ^{[6
]}

Yun, Sung-Hwan ^{[7
]}

Lim, Jae Yun ^{[1
,2
,3
]}

Kim, Minkyun ^{[1
,2
,3
]}

Lee, Yong-Hwan ^{[1
,2
,3
]}

Choi, Yang-Do ^{[1
,2
,3
]}

Lee, Yin-Won ^{[1
,2
,3
]}

机构：

[1] Seoul Natl Univ, Dept Agr Biotechnol, Seoul, South Korea

[2] Seoul Natl Univ, Ctr Fungal Pathogenesis, Seoul, South Korea

[3] Seoul Natl Univ, Ctr Agr Biomat, Seoul, South Korea

[4] Dong A Univ, Dept Appl Biol, Pusan, South Korea

[5] Zhengzhou Tobacco Res Inst, China Tobacco Gene Res Ctr, Zhengzhou, Henan, Peoples R China

[6] Korea Res Inst Chem Technol, Chem Biotechnol Ctr, Taejon, South Korea

[7] Soonchunhyang Univ, Dept Med Biotechnol, Asan, South Korea

来源：

PLOS PATHOGENS | 2011年 / 7卷 / 10期

基金：

新加坡国家研究基金会;

关键词：

GIBBERELLA-ZEAE; GENE-EXPRESSION; SUBMERGED CULTURES; REDUCED VIRULENCE; GENOME; REVEALS; PATHOGENICITY; ZEARALENONE; METABOLISM; INFECTION;

D O I：

10.1371/journal.ppat.1002310

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Fusarium graminearum is an important plant pathogen that causes head blight of major cereal crops. The fungus produces mycotoxins that are harmful to animal and human. In this study, a systematic analysis of 17 phenotypes of the mutants in 657 Fusarium graminearum genes encoding putative transcription factors (TFs) resulted in a database of over 11,000 phenotypes (phenome). This database provides comprehensive insights into how this cereal pathogen of global significance regulates traits important for growth, development, stress response, pathogenesis, and toxin production and how transcriptional regulations of these traits are interconnected. In-depth analysis of TFs involved in sexual development revealed that mutations causing defects in perithecia development frequently affect multiple other phenotypes, and the TFs associated with sexual development tend to be highly conserved in the fungal kingdom. Besides providing many new insights into understanding the function of F. graminearum TFs, this mutant library and phenome will be a valuable resource for characterizing the gene expression network in this fungus and serve as a reference for studying how different fungi have evolved to control various cellular processes at the transcriptional level.

引用

页数：10

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