Tn5060 from the Siberian permafrost is most closely related to the ancestor of Tn21 prior to integron acquisition

被引：24

作者：

Kholodii, G ^{[1
]}

Mindlin, S ^{[1
]}

Petrova, M ^{[1
]}

Minakhina, S ^{[1
]}

机构：

[1] Russian Acad Sci, Inst Mol Genet, Moscow 123182, Russia

来源：

FEMS MICROBIOLOGY LETTERS | 2003年 / 226卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

integron In2; mercury resistance operon; Tn21; subgroup; TnX; transposon evolution;

D O I：

10.1016/S0378-1097(03)00559-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A Tn21-related mercury resistance transposon, Tn5060, has been isolated from Pseudomonas sp. strain A19-1 from a 8000-10000-year-old Siberian permafrost sample, and sequenced. Like Tn21, the element transposes to different plasmids at a frequency of 10(-2)-10(-3) per target plasmid transfer. Comparison of the complete Tn5060 DNA sequence (8667 bp) with that of Tn21 (19 672 bp) shows that Tn5060 does not contain integron In2 and deviates from Tn21 in four nucleotide positions. These and other comparative data demonstrate that Tn5060 is the most closely related of the characterized mercury resistances to the as yet hypothetical immediate ancestor of Tn21, TnX. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：251 / 255

页数：5

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