Trade in Zambian Edible Orchids-DNA Barcoding Reveals the Use of Unexpected Orchid Taxa for Chikanda

被引：7

作者：

Veldman, Sarina ^{[1
]}

Kim, Seol-Jong ^{[1
]}

van Andel, Tinde R. ^{[2
]}

Font, Maria Bello ^{[3
]}

Bone, Ruth E. ^{[4
]}

Bytebier, Benny ^{[5
]}

Chuba, David ^{[6
]}

Gravendeel, Barbara ^{[2
,7
,8
]}

Martos, Florent ^{[5
,9
]}

Mpatwa, Geophat ^{[10
]}

Ngugi, Grace ^{[5
,11
]}

Vinya, Royd ^{[10
]}

Wightman, Nicholas ^{[12
]}

Yokoya, Kazutoma ^{[4
]}

de Boer, Hugo J. ^{[1
,2
]}

机构：

[1] Uppsala Univ, Dept Organismal Biol, Systemat Biol, Norbyvagen 18D, S-75236 Uppsala, Sweden

[2] Naturalis Biodivers Ctr, POB 9517, NL-2300 RA Leiden, Netherlands

[3] Univ Oslo, Nat Hist Museum, Postboks 1172, N-0318 Oslo, Norway

[4] Royal Bot Gardens, Richmond TW9 3AB, Surrey, England

[5] Univ KwaZulu Natal, Sch Life Sci, Bews Herbarium, Pr Bag X01, ZA-3209 Scottsville, South Africa

[6] Univ Zambia, Dept Biol Sci, Box 32379, Lusaka, Zambia

[7] Leiden Univ, Inst Biol Leiden, POB 9505, NL-2300 RA Leiden, Netherlands

[8] Univ Appl Sci Leiden, Zernikedreef 11, NL-2333 CK Leiden, Netherlands

[9] Sorbonne Univ, Museum Natl Hist Nat, CNRS, Inst Systemat Evolut Biodiversite ISYEB,EPHE, CP50,45 Rue Buffon, F-75005 Paris, France

[10] Copperbelt Univ, Sch Nat Resources, POB 21692, Kitwe, Zambia

[11] Natl Museums Kenya, East African Herbarium, POB 40658-00100, Nairobi, Kenya

[12] Homegarden Landscape Consultants Ltd, P Bag 30C, Lusaka, Zambia

来源：

GENES | 2018年 / 9卷 / 12期

基金：

瑞典研究理事会;

关键词：

CITES; chikanda; conservation; DNA barcoding; orchids; species identification; CONCERTED EVOLUTION; SEQUENCES; NUCLEAR; MATK;

D O I：

10.3390/genes9120595

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

In Zambia, wild edible terrestrial orchids are used to produce a local delicacy called chikanda, which has become increasingly popular throughout the country. Commercialization puts orchid populations in Zambia and neighbouring countries at risk of overharvesting. Hitherto, no study has documented which orchid species are traded on local markets, as orchid tubers are difficult to identify morphologically. In this study, the core land-plant DNA barcoding markers rbcL and matK were used in combination with nrITS to determine which species were sold in Zambian markets. Eighty-two interviews were conducted to determine harvesting areas, as well as possible sustainability concerns. By using nrITS DNA barcoding, a total of 16 orchid species in six different genera could be identified. Both rbcL and matK proved suitable to identify the tubers up to the genus or family level. Disa robusta, Platycoryne crocea and Satyrium buchananii were identified most frequently and three previously undocumented species were encountered on the market. Few orchid species are currently listed on the global International Union for the Conservation of Nature (IUCN) Red List. Local orchid populations and endemic species could be at risk of overharvesting due to the intensive and indiscriminate harvesting of chikanda orchids, and we therefore encourage increased conservation assessment of terrestrial African orchids.

引用

页数：15