Boosting the down-shifting luminescence of rare-earth nanocrystals for biological imaging beyond 1500 nm

被引：481

作者：

Zhong, Yeteng ^{[1
,2
]}

Ma, Zhuoran ^{[2
]}

Zhu, Shoujun ^{[2
]}

Yue, Jingying ^{[2
]}

Zhang, Mingxi ^{[2
]}

Antaris, Alexander L. ^{[2
]}

Yuan, Jie ^{[2
]}

Cui, Ran ^{[2
]}

Wan, Hao ^{[2
]}

Zhou, Ying ^{[2
]}

Wang, Weizhi ^{[1
,2
]}

Huang, Ngan F. ^{[3
]}

Luo, Jian ^{[4
]}

Hu, Zhiyuan ^{[1
,5
,6
]}

Dai, Hongjie ^{[2
]}

机构：

[1] Natl Ctr Nanosci & Technol China, CAS Ctr Excellence Nanosci, CAS Key Lab Standardizat & Measurement Nanotechno, CAS Key Lab Biomed Effects Nanomat & Nanosafety, Beijing 100190, Peoples R China

[2] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[3] Stanford Univ, Stanford Cardiovasc Inst, Stanford, CA 94305 USA

[4] Stanford Univ, Dept Neurol & Neurol Sci, Sch Med, Stanford, CA 94305 USA

[5] Univ Chinese Acad Sci, Sinodanish Coll, Beijing 100049, Peoples R China

[6] Yangtze River Delta Acad Nanotechnol & Ind Dev Re, Jiaxing 314000, Zhejiang, Peoples R China

来源：

NATURE COMMUNICATIONS | 2017年 / 8卷

基金：

美国国家卫生研究院; 中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

NEAR-INFRARED WINDOW; UP-CONVERSION; ENERGY MIGRATION; QUANTUM DOTS; NANOPARTICLES; DYE; FLUOROPHORES; EMISSION; PHOTOLUMINESCENCE; BRIGHT;

D O I：

10.1038/s41467-017-00917-6

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In vivo fluorescence imaging in the near-infrared region between 1500-1700 nm (NIR-IIb window) affords high spatial resolution, deep-tissue penetration, and diminished auto-fluorescence due to the suppressed scattering of long-wavelength photons and large fluorophore Stokes shifts. However, very few NIR-IIb fluorescent probes exist currently. Here, we report the synthesis of a down-conversion luminescent rare-earth nanocrystal with cerium doping (Er/Ce co-doped NaYbF4 nanocrystal core with an inert NaYF4 shell). Ce doping is found to suppress the up-conversion pathway while boosting down-conversion by similar to 9-fold to produce bright 1550 nm luminescence under 980 nm excitation. Optimization of the inert shell coating surrounding the core and hydrophilic surface functionalization minimize the luminescence quenching effect by water. The resulting biocompatible, bright 1550 nm emitting nanoparticles enable fast in vivo imaging of blood vasculature in the mouse brain and hindlimb in the NIR-IIb window with short exposure time of 20 ms for rare-earth based probes.

引用

页数：7

共 49 条

[31] Novel benzo-bis(1,2,5-thiadiazole) fluorophores for in vivo NIR-II imaging of cancer [J].