Comparative study of the chiral resolution of β-blockers on cellulose tris (3,5-dimethylphenylcarbamate) phases in normal and reversed phase modes

The chiral separation of beta -blockers on cellulose tris (3,5-dimethylphenylearbamate) columns is described. The separation power of a Chiracel OD normal phase column, a Chiracel OD-RH reversed phase column, and a Chiracel ODRH reversed phase narrow-bore column were compared. Hexane-2-propanol-diethylamine mixtures were used as mobile phase in the normal phase mode and sodium perchlorate buffer containing different amounts of acetonitrile in the reversed phase mode. Sixteen mu -blockers were resolved under normal phase conditions and eleven under reversed phase conditions. The values of a and Rs on normal phase column ranged from 4.46 to 1.10 and 10.04 to 0.97, respectively. The values of a and Rs on reversed phase columns varied from 4.04 to 1.04 and 3.98 to 0.40 (on normal bore column) and 4.36 to 1.06 and 2.26 to 0.40 (on narrow bore column).