Enumeration of viable Enterococcus faecalis, a predominant apical periodontitis pathogen, using propidium monoazide and quantitative real-time polymerase chain reaction

被引：12

作者：

Takahashi, Yusuke

Yoshida, Akihiro ^{[1
]}

Nagayoshi, Masato ^{[2
]}

Kitamura, Chiaki ^{[2
]}

Nishihara, Tatsuji ^{[3
]}

Awano, Shuji

Ansai, Toshihiro

机构：

[1] Kyushu Dent Coll, Div Community Oral Hlth Sci, Kokurakita Ku, Kitakyushu, Fukuoka 8038580, Japan

[2] Kyushu Dent Coll, Div Pulp Biol Operat Dent & Endodont, Kitakyushu, Fukuoka 8038580, Japan

[3] Kyushu Dent Coll, Div Infect & Mol Biol, Kitakyushu, Fukuoka 8038580, Japan

来源：

MICROBIOLOGY AND IMMUNOLOGY | 2011年 / 55卷 / 12期

关键词：

apical periodontitis; Enterococcus faecalis; propidium monoazide; viable cell; ETHIDIUM MONOAZIDE; DEAD CELLS; PCR ASSAY; POPULATION; CANALS; DNA;

D O I：

10.1111/j.1348-0421.2011.00390.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To discriminate between viable and non-viable Enterococcus faecalis, the predominant pathogen in apical periodontitis, a real-time PCR method combined with propidium monoazide (PMA) was developed and evaluated. PMA had no antimicrobial effect on E. faecalis cells and permitted enumeration of both viable and non-viable cells. Therefore, E. faecalis cells from the root canals of nine patients with apical periodontitis were analyzed to evaluate the diagnostic usefulness of this approach. Viable and non-viable E. faecalis cells were successfully discriminated in these clinical specimens. A real-time PCR assay combined with PMA will contribute to the precise diagnosis of apical periodontitis.

引用

页码：889 / 892

页数：4

共 12 条

[1]

Bonten M J, 2001, Lancet Infect Dis, V1, P314, DOI 10.1016/S1473-3099(01)00145-1

[2] Molecular cloning, overexpression, purification, and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalis [J].