The effect of vitronectin on the differentiation of embryonic stem cells in a 3D culture system

被引：42

作者：

Heydarkhan-Hagvall, Sepideh ^{[1
]}

Gluck, Jessica M. ^{[1
]}

Delman, Connor ^{[1
]}

Jung, Monica ^{[1
]}

Ehsani, Nazanin ^{[1
]}

Full, Sean ^{[1
]}

Shemin, Richard J. ^{[1
]}

机构：

[1] Univ Calif Los Angeles, David Geffen Sch Med, Cardiovasc Tissue Engn Lab, Dept Surg, Los Angeles, CA 90095 USA

来源：

BIOMATERIALS | 2012年 / 33卷 / 07期

关键词：

Extracellular matrix; Niche; Cardiovascular tissue engineering; Stem cell; Scaffold; EXTRACELLULAR-MATRIX; SMOOTH-MUSCLE; PROGENITOR CELLS; GROWTH-FACTORS; IN-VITRO; PROTEIN; FIBRONECTIN; ADSORPTION; EXPRESSION; LINEAGES;

D O I：

10.1016/j.biomaterials.2011.11.065

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

While stem cell niches in vivo are complex three-dimensional (3D) microenvironments, the relationship between the dimensionality of the niche to its function is unknown. We have created a 3D microenvironment through electrospinning to study the impact of geometry and different extracellular proteins on the development of cardiac progenitor cells (Flk-1(+)) from resident stem cells and their differentiation into functional cardiovascular cells. We have investigated the effect of collagen IV, fibronectin, laminin and vitronectin on the adhesion and proliferation of murine ES cells as well as the effects of these proteins on the number of Flk-1(+) cells cultured in 2D conditions compared to 3D system in a feeder free condition. We found that the number of Flk-1(+) cells was significantly higher in 3D scaffolds coated with laminin or vitronectin compared to colIV-coated scaffolds. Our results show the importance of defined culture systems in vitro for studying the guided differentiation of pluripotent embryonic stem cells in the field of cardiovascular tissue engineering and regenerative medicine. Published by Elsevier Ltd.

引用

页码：2032 / 2040

页数：9

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