Can maternal exposure to tamoxifen compromise sperm and behavioural parameters of male rat offspring?

被引:6
作者
Manoel, Beatriz de Matos [1 ]
Moreira, Suyane da Silva [2 ]
Zampieri, Gabriela Morelli [2 ]
Pinheiro, Luisa Machado [2 ]
Jorge, Barbara Campos [2 ]
Casali Reis, Ana Carolina [2 ]
Leite Kassuya, Candida Aparecida [1 ]
Arena, Arielle Cristina [2 ,3 ]
机构
[1] Univ Fed Grande Dourados UFGD, Fac Hlth Sci, Dourados, MS, Brazil
[2] Univ Estadual Paulista UNESP, Inst Biosci Botucatu, Dept Struct & Funct Biol, Dist Rubiao Jr S-N,Caixa Postal 510, BR-18618970 Botucatu, SP, Brazil
[3] Univ Estadual Paulista UNESP, Inst Biosci Botucatu, Ctr Toxicol Assistance CEATOX, Sao Paulo, Brazil
基金
巴西圣保罗研究基金会;
关键词
Selective estrogen receptor modulator; brain sexual differentiation; sexual development; epididymis; reproductive parameters; ESTROGEN-RECEPTOR-ALPHA; ANOGENITAL DISTANCE; SEXUAL-DIFFERENTIATION; SOCIAL PLAY; IN-UTERO; PREGNANCY; ANDROGEN; TESTOSTERONE; EPIDIDYMIS; MOTILITY;
D O I
10.1016/j.reprotox.2021.12.010
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Tamoxifen, a selective non-steroidal estrogen receptor modulator, is the standard adjuvant endocrine treatment for breast cancer. Since information on the risk of using tamoxifen during pregnancy is still scarce, this study evaluated whether the in utero and lactational treatment with this drug could compromise reproductive and behavioural parameters in male offspring. Pregnant Wistar rats were exposed to three doses of tamoxifen (0.12; 0.6; 3 mu g/kg), by gavage, from gestational day 15 to lactational day 20. Tamoxifen exposure did not alter the anogenital distance in the male offspring; however, there was a significant increase in the body weight in the 0.12 mu g/kg dose and a decrease in the 0.6 mu g/kg dose. The male offspring treated with the highest dose exhibited a delay in the onset of puberty, evidenced by an increase in the age of preputial separation. Regarding sperm parameters, there was an increase in the sperm count in the cauda epididymis in the intermediate and highest dose groups, in addition to an increase in the number of static sperm and a decrease in the progressive sperm in the same groups. Moreover, an increase in the number of hyperplasia of the epithelial clear cells was observed in the epididymis. In conclusion, the present study demonstrated that maternal exposure to tamoxifen compromised the installation of puberty of the male offspring and the maturation of the epididymis, affecting sperm storage and motility in the adult life.
引用
收藏
页码:1 / 9
页数:9
相关论文
共 57 条
[1]  
AAFJES JH, 1980, J REPROD FERTIL, V58, P345
[2]   Lack of opioid or dopaminergic effects on unconditioned sexual incentive motivation in male rats [J].
Ågmo, A .
BEHAVIORAL NEUROSCIENCE, 2003, 117 (01) :55-68
[3]   Male rat sexual behavior [J].
Agmo, A .
BRAIN RESEARCH PROTOCOLS, 1997, 1 (02) :203-209
[4]   Tamoxifen-loaded poly(L-lactide) nanoparticles: Development, characterization and in vitro evaluation of cytotoxicity [J].
Altmeyer, Clescila ;
Karam, Thaysa Ksiaskiewcz ;
Khalil, Najeh Maissar ;
Mainardes, Rubiana Mara .
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2016, 60 :135-142
[5]   Maternal exposure to ibuprofen can affect the programming of the hypothalamus of the male offspring [J].
Balin, Paola da Silva ;
Jorge, Barbara Campos ;
Ribeiro Leite, Andressa Rejani ;
Borges, Cibele Santos ;
Oba, Eunice ;
Ramo Silva, Erick Jose ;
de Barros, Aline Lima ;
Castro Horta-Junior, Jose de Anchieta ;
Arena, Arielle Cristina .
REGULATORY TOXICOLOGY AND PHARMACOLOGY, 2020, 111
[6]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[7]   The Development of Female Sexual Behavior Requires Prepubertal Estradiol [J].
Brock, Olivier ;
Baum, Michael J. ;
Bakker, Julie .
JOURNAL OF NEUROSCIENCE, 2011, 31 (15) :5574-5578
[8]  
Cho H. Wook, 2003, ANTIESTROGEN ICI 182
[9]  
Cury Carlos A., 2006, Int. braz j urol., V32, P211
[10]  
Danielsson Bengt R, 2013, Methods Mol Biol, V947, P311, DOI 10.1007/978-1-62703-131-8_24