In Vivo Cellular MRI of Dendritic Cell Migration Using Micrometer-Sized Iron Oxide (MPIO) Particles

被引：29

作者：

Rohani, Roja ^{[1
,2
]}

de Chickera, Sonali N. ^{[3
,4
]}

Willert, Christy ^{[4
]}

Chen, Yuhua ^{[2
]}

Dekaban, Gregory A. ^{[4
,5
]}

Foster, Paula J. ^{[1
,2
]}

机构：

[1] Univ Western Ontario, Dept Med Biophys, London, ON, Canada

[2] Univ Western Ontario, Robarts Res Inst, Imaging Res Labs, London, ON, Canada

[3] Univ Western Ontario, Dept Anat & Cell Biol, London, ON, Canada

[4] Univ Western Ontario, Robarts Res Inst, BioTherapeut Res Labs, London, ON, Canada

[5] Univ Western Ontario, Dept Microbiol & Immunol, Robarts Res Inst, London, ON, Canada

来源：

MOLECULAR IMAGING AND BIOLOGY | 2011年 / 13卷 / 04期

关键词：

MRI; Dendritic cells; Cancer; Immunotherapy; Iron oxide; Contrast agent; MPIO; LYMPH-NODES; MELANOMA PATIENTS; T-CELLS; SINGLE CELLS; MOUSE-BRAIN; SPINAL-CORD; VACCINATION; TRACKING; MACROPHAGES; MATURATION;

D O I：

10.1007/s11307-010-0403-0

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

This study seeks to assess the use of labeling with micron-sized iron oxide (MPIO) particles for the detection and quantification of the migration of dendritic cells (DCs) using cellular magnetic resonance imaging (MRI). DCs were labeled with red fluorescent MPIO particles for detection by cellular MRI and a green fluorescent membrane dye (PKH67) for histological detection. MPIO-labeled DCs or unlabeled control DCs were injected into mice footpads at two doses (0.1 x 10(6) and 1 x 10(6)). Images were acquired at 3 Tesla before DC injection and 2, 3, and 7 days post-DC injection. Labeling DCs with MPIO particles did not affect viability, but it did alter markers of DC activation and maturation. MRI and fluorescence microscopy allowed for the detection of MPIO-labeled DCs within the draining popliteal nodes after their injection into the footpad. This paper presents the first report of the successful use of fluorescent MPIO particles to label and track DC migration.

引用

页码：679 / 694

页数：16

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