Logic and Reversible Dual DNA Detection Based on the Assembly of Graphene Oxide and DNA-templated Quantum Dots

We prepared water-soluble monolayer graphene oxide (GO) and DNA-templated CdTe quantum dots (P1), which were further assembled via pi-pi stacking interaction to generate nano-biosensors for the logical detection of dual DNA molecules with high selectivity. Moreover, reversible cyclic dual DNA detection is realized by changing DNA sequences, which enables regeneration of the biosensors. A variety of instruments were used to characterize biosensor preparation and detection process including atomic force microscopy (AFM), transmission electron microscopy (TEM), electrophoresis, fluorescence spectroscopy and so on. The prepared nano-biosensor holds great promise for nucleic acid detection.