DNA integrity as biomarker in pancreatic cyst fluid

被引：0

作者：

Utomo, Wesley K. ^{[1
]}

Janmaat, Vincent T. ^{[1
]}

Verhaar, Auke P. ^{[1
]}

Cros, Jerome ^{[2
]}

Levy, Philippe ^{[3
]}

Ruszniewski, Philippe ^{[3
]}

Vredenbregt-van den Berg, Mirella S. ^{[4
]}

Jenster, Guido ^{[4
]}

Bruno, Marco J. ^{[1
]}

Braat, Henri ^{[1
]}

Fuhler, Gwenny M. ^{[1
]}

Peppelenbosch, Maikel P. ^{[1
]}

机构：

[1] Erasmus MC Univ, Med Ctr, Dept Gastroenterol & Hepatol, Rotterdam, Netherlands

[2] Univ Paris Diderot, Dept Pathol, Hop Beaujon, INSERM,U1149, Clichy, France

[3] Univ Denis Diderot Paris VII, Hop Beaujon, Pole Malad Appareil Digestif, Serv Pancreatol Gastroenterol, Clichy, France

[4] Erasmus MC Univ, Med Ctr, Dept Urol, Rotterdam, Netherlands

来源：

AMERICAN JOURNAL OF CANCER RESEARCH | 2016年 / 6卷 / 08期

关键词：

Cell free DNA; circulating; serum; pancreatic cyst fluid; PCR; ALU repeats; CELL-FREE DNA; PROSTATE-CANCER; CIRCULATING DNA; BREAST-CANCER; BLOOD-PLASMA; MARKER;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Identification of pancreatic cysts with malignant potential is important to prevent pancreatic cancer development. Integrity of cell free DNA (cfDNA) has been described as tumor biomarker, but its potential for pancreatic cancer is unclear. While normal apoptotic cells release uniformly truncated DNA, malignant tissues release long fragments of cell free DNA (cfDNA). We measured 247 base pair (bp) and 115 bp DNA fragments of ALU repeats by qPCR in serum from healthy controls and pancreatic cancer patients, and in cyst fluid from pancreatic cyst patients. No differences in total cfDNA (ALU115) and cfDNA integrity (ALU247/115) were observed between sera from healthy controls (n=19) and pancreatic cancer patients (n=19). Although elevated as compared to serum, but no differences in cfDNA were found in cyst fluid from high risk (n=10) and low risk (n=20) cyst patients. We conclude that cfDNA integrity is not a useful marker to identify (pre) malignant pancreatic lesions.

引用

页码：1837 / 1841

页数：5

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