Multivariable parameter optimization for the endoglucanase production by Trichoderma reesei Rut C30 from Ocimum gratissimum seed

被引：24
|
作者：
Das, Mithu [1 ]
Banerjee, Rintu [1 ]
Bal, Satish [1 ]
机构：
[1] Indian Inst Technol, Agr & Food Engn Dept, Microbial Biotechnol & Downstream Proc Lab, Kharagpur 721302, W Bengal, India
来源：
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY | 2008年 / 51卷 / 01期
关键词：
enzyme; endoglucanase; carboxymethylcellulase (CMCase); EVOP factorial design technique;
D O I：
10.1590/S1516-89132008000100005
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The aim of this study was to evaluate the interaction effects of the physico-chemical parameters on the endoglucanase (CMCase) production by Trichoderma reesei Rut C30 on a cellulosic agro-residue by the solid-state fermentation (SSF) and to determine their optimum values by the EVOP factorial design technique. The best combination of physical parameters for the maximum production of the endoglucanase (CMCase) was 28 degrees C temperature, 79% relative humidity and 4.8 pH of the medium. The best combination of the chemical parameters was (mg/L) nicotinic acid 15, naphthalene acetic acid 7, ferric chloride 5 and Tween-80 6. With the application of this technique, the yield of the CMCase increased by similar to 2.3 fold.
引用
收藏
页码：35 / 41
页数：7
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