Effects of Bacitracin and Bacitracin Zinc on &ITIn Vitro&IT Fermentation, methane Production and microbial Populations of the Rumen

被引：0

作者：

Chen, Y. Y. ^{[1
]}

Shen, J. S. ^{[1
]}

Liu, Z. ^{[1
]}

Lv, P. A. ^{[1
]}

Zhu, W. Y. ^{[1
]}

机构：

[1] Nanjing Agr Univ, Jiangsu Key Lab Gastrointestinal Nutr & Anim Hlth, Coll Anim Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China

来源：

ANIMAL NUTRITION AND FEED TECHNOLOGY | 2017年 / 17卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Ammonia; Bacitracin zinc; Methane; Microbial population; Rumen fermentation; RUMINAL FERMENTATION; AMINO-ACIDS; DAIRY-COWS; ANTIBIOTICS; METABOLISM; GROWTH; ASSAY;

D O I：

10.5958/0974-181X.2017.00029.4

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

This study was conducted to investigate the effects of bacitracin (B) and bacitracin zinc (BZ) on in vitro fermentation, methane and ammonia production, and select microbial populations of the rumen. Both B and BZ were added at 3 doses, namely 10 (low), 30 (medium), and 90 (high) mg/l of in vitro fermentation medium. Results showed that B and BZ had similar effects on rumen fermentation characteristics and microbial populations. Low dosage of B or BZ addition reduced (P < 0.05) methane and ammonia production, while had no adverse effects (P > 0.05) on total VFA concentration and feed digestibility. The population of total bacteria was unaffected (P > 0.05) by low B and BZ doses, but was significantly (P < 0.05) decreased by high B and BZ doses. All B and BZ doses lowered (P < 0.05) the populations of Firmicute, Bacteroidetes, methanogens, Clostridium aminophilum and Clostridium sticklandii. However, B and BZ addition had no influence (P > 0.05) on the populations of protozoa and fungi. Results indicated that optimum B or BZ addition could reduce methane and ammonia production, with no adverse effects on total VFA concentration and feed digestibility. Further, these effects are probably associated with the variation of rumen functional microbial populations.

引用

页码：303 / 314

页数：12

共 28 条

[1]

[Anonymous], 2006, LIVESTOCKS LONG SHAD

[2]

CHANEY AL, 1962, CLIN CHEM, V8, P130

[3]

Cogliani C., 2011, Microbe, V6, P274, DOI DOI 10.1128/MICROBE.6.274.1

[4] Utilization of amino acids by bacteria from the pig small intestine [J].

Dai, Zhao-Lai ;

Zhang, Jing ;

Wu, Guoyao ;

Zhu, Wei-Yun .

AMINO ACIDS, 2010, 39 (05) :1201-1215

[5] Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound bromochloromethane [J].

Denman, Stuart E. ;

Tomkins, NigelW. ;

McSweeney, Christopher S. .

FEMS MICROBIOLOGY ECOLOGY, 2007, 62 (03) :313-322

[6] Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen [J].

Denman, Stuart E. ;

McSweeney, Christopher S. .

FEMS MICROBIOLOGY ECOLOGY, 2006, 58 (03) :572-582

[7] Ammonia production by ruminal microorganisms and enumeration, isolation, and characterization of bacteria capable of growth on peptides and amino acids from the sheep rumen [J].

Eschenlauer, SCP ;

McKain, N ;

Walker, ND ;

McEwan, NR ;

Newbold, CJ ;

Wallace, RJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (10) :4925-4931

[8] Ruminal nitrogen metabolism: Perspectives for integration of microbiology and nutrition for dairy [J].

Firkins, J. L. ;

Yu, Z. ;

Morrison, M. .

JOURNAL OF DAIRY SCIENCE, 2007, 90 :E1-E16

[9] A comparison of rumen fluid sampled by oro-ruminal probe versus rumen fistula [J].

Geishauser, T ;

Gitzel, A .

SMALL RUMINANT RESEARCH, 1996, 21 (01) :63-69

[10] Real-time PCR quantification of the predominant bacterial divisions in the distal gut of Meishan and Landrace pigs [J].

Guo, Xiulan ;

Xia, Xinjie ;

Tang, Renyong ;

Wang, Kangning .

ANAEROBE, 2008, 14 (04) :224-228

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