Stereoselective reduction of alkyl 3-oxobutanoate by carbonyl reductase from Candida magnoliae

被引：71
作者：
Yasohara, Y
Kizaki, N
Hasegawa, J
Wada, M
Kataoka, M
Shimizu, S
机构：
[1] Kaneka Corp, Fine Chem Res Labs, Takasago, Hyogo 6768688, Japan
[2] Fukui Prefectural Chem, Dept Biosci, Fukui 9101195, Japan
[3] Kyoto Univ, Grad Sch Agr, Div Appl Life Sci, Sakyo Ku, Kyoto 6068502, Japan
来源：
TETRAHEDRON-ASYMMETRY | 2001年 / 12卷 / 12期
关键词：
D O I：
10.1016/S0957-4166(01)00279-8
中图分类号：
O61 [无机化学];
学科分类号：
070301 ; 081704 ;
摘要：
The enantioselective reduction of alkyl 3-oxobutanoates by carbonyl reductase (Sl) from Candida magnoliae was investigated. S1 reduced alkyl 4-halo-3-oxobutanoates to the corresponding enantiomerically pure (S)-3-hydroxy esters. Escherichia coli HB101 transformant co-overproducing the S1 and glucose dehydrogenase from Bacillus megaterium, produced optically pure alkyl 4-substituted-3-hydroxybutanoates in a two-phase water/organic solvent system. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：1713 / 1718
页数：6
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