Preparation of konjac glucomannan hydrogels as DNA-controlled release matrix

被引：35

作者：

Wen, Xian ^{[1
]}

Wang, Ting ^{[1
]}

Wang, Zhiyong ^{[1
]}

Li, Li ^{[1
]}

Zhao, Changsheng ^{[1
]}

机构：

[1] Sichuan Univ, Coll Polymer Sci & Engn, State Key Lab Polymer Mat Engn, Natl Engn Res Ctr Biomat, Chengdu 610065, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2008年 / 42卷 / 03期

基金：

中国国家自然科学基金;

关键词：

hydrogel; konjac glucomannan; controlled release; DNA delivery;

D O I：

10.1016/j.ijbiomac.2007.11.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, hydrogels for DNA-controlled release was prepared with konjac glucomannan (KGM), a water-soluble non-ionic polysaccharide, by means of deacetylated reaction and physically cross-linking method under mild conditions. The properties of the KGM hydrogels were analyzed by FTIR spectra and scanning electron microscopy (SEM). The integrality of the released DNA was investigated by circular dichroism (CD). The DNA release kinetics was performed using the DNA-loaded KGM gels in buffer solutions of pH 7.4 at 37 +/- 0.5 degrees C. Peppas model and Higuchi model were used to analysis the DNA release mechanism; the data indicated that the DNA release can be controlled by changing the preparation conditions and the structure parameters of the gels. This study suggested that the KGM hydrogels have a potential use for advanced controlled release. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：256 / 263

页数：8

共 33 条

[1] RELEASE MECHANISMS IN GELFORMING SUSTAINED-RELEASE PREPARATIONS [J].