Identification of Proteins Using Four Methods of Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

被引：5

作者：

Bendinskas, Kestutis G. ^{[1
]}

Canning, Ashley J. ^{[1
]}

Smith, Ashlee E. ^{[1
]}

机构：

[1] SUNY Coll Oswego, Dept Chem, 360 Shineman Ctr,30 Centennial Dr, Oswego, NY 13126 USA

来源：

JOURNAL OF CHEMICAL EDUCATION | 2019年 / 96卷 / 11期

关键词：

Upper-Division Undergraduate; Graduate Education/Research; Hands-On Learning/Manipulatives; Biochemistry; Analytical Chemistry; Laboratory Instruction; Bioanalytical Chemistry; Biotechnology; Mass Spectrometry; MERCURY;

D O I：

10.1021/acs.jchemed.8b00687

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An upper-division undergraduate- or graduate-level laboratory experiment, challenging students to identify bovine serum albumin, equine myoglobin, bovine calmodulin, and bovine cytochrome c, uses four methods of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS): linear positive, in-source decay, peptide mass fingerprint, and postsource decay. Individual hands-on experience with each method is provided in a three week experiment. The importance of sequencing proteins/peptides when identifying proteomic targets using MALDI-TOF MS is emphasized.

引用

页码：2600 / 2605

页数：11

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