1H NMR metabolomics of earthworm responses to polychlorinated biphenyl (PCB) exposure in soil

被引:37
作者
Aslund, Melissa L. Whitfield [1 ]
Simpson, Andre J. [1 ]
Simpson, Myrna J. [1 ]
机构
[1] Univ Toronto, Dept Phys & Environm Sci, Toronto, ON M1C 1A4, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
Persistent organic pollutants (POPs); Metabonomics; Pearson correlation; Risk assessment; Bioavailability; Sub-lethal toxicity; NMR-BASED METABOLOMICS; CROSS-VALIDATION; EISENIA-FOETIDA; RISK-ASSESSMENT; BIOMARKER; IDENTIFICATION; METABONOMICS; BIOAVAILABILITY; CONTAMINATION; NORMALIZATION;
D O I
10.1007/s10646-011-0638-9
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
H-1 NMR-based metabolomics was used to examine the metabolic profile of D2O-buffer extracted tissues of Eisenia fetida earthworms exposed for 2 days to an artificial soil spiked with sub-lethal concentrations of polychlorinated biphenyls (PCBs) (0, 0.5, 1, 5, 10, or 25 mg/kg Aroclor 1254). Univariate statistical analysis of the identified metabolites revealed a significant increase in ATP concentration in earthworms exposed to the highest soil PCB concentration, but detected no significant changes in other metabolites. However, a multivariate approach which considers alterations in multiple metabolites simultaneously, identified a significant linear relationship between earthworm metabolic profiles and PCB concentration (cross-validated PLS-regression with 7 components, (RX)-X-2 = 0.99, (RY)-Y-2 = 0.77, Q(2)Y = 0.45, P < 0.001). Significant changes in pair-wise metabolic correlations were also detected as PCB concentration increased. For example, lysine and ATP concentrations showed no apparent correlation in control earthworms (r = 0.22, P = 0.54), but were positively correlated in earthworms from the 25 mg/kg treatment (r = 0.87, P = 0.001). Overall, the observed metabolic responses suggest that PCBs disrupted both carbohydrate (energy) metabolism and membrane (osmolytic) function in E. fetida. The ability of H-1 NMR-based metabolomics to detect these responses suggests that this method offers significant potential for direct assessment of sub-lethal PCB toxicity in soil.
引用
收藏
页码:836 / 846
页数:11
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