Agrobacterium tumefaciens Mediated Transformation of rolC::Hd3a-GFP in Black Rice (Oryza sativa L. cv. Cempo Ireng) to Promote Early Flowering

被引：4

作者：

Purwestri, Yekti Asih ^{[1
,2
]}

Sari, Resta Dewi Komala ^{[1
]}

Anggraeni, Lisa Novita ^{[2
]}

Sasongko, Aries Bagus ^{[2
]}

机构：

[1] Univ Gadjah Mada, Res Ctr Biotechnol, Barek Yogyakarta 55281, Indonesia

[2] Univ Gadjah Mada, Fac Biol, Yogyakarta 55281, Indonesia

来源：

2nd Humboldt Kolleg in conjunction with International Conference on Natural Sciences 2014, HK-ICONS 2014 | 2015年 / 14卷

关键词：

Black rice (Oryza sativa L. cv. Cempo Ireng); Hd3a; hpt; 2N(6) medium; 2,4-dichlorophenoxyacetic acid; Agrobacterium-mediated transformation;

D O I：

10.1016/j.proche.2015.03.063

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The purpose of this study was to transform rolC:: Hd3a-GFP by Agrobacterium tumefaciens on black rice (Oryza sativa L. cv. Cempo Ireng). The callus formation were induced from scutellum using two different media, 2N(6) and 2,4-dichlorophenoxyacetic acid (2,4-D). PCR analysis using specific primers for Hd3a and hpt was performed to determine the stability of rolC:: Hd3a-GFP construct before co-cultivation of callus and Agrobacterium tumefaciens. This study indicated that black rice callus induction on 2N6 medium responded faster than that on MS 2,4-D medium and generated friable calli for Agrobaterium-mediated transformation. The screening result showed that several calli were competent to regenerate on medium containing hygromysin and demonstrated Hd3a insertion gene. (C) 2015 The Authors. Published by Elsevier B.V.

引用

页码：469 / 473

页数：5

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