TNFα enhances the motility and invasiveness of prostatic cancer cells by stimulating the expression of selective glycosyl- and sulfotransferase genes involved in the synthesis of selectin ligands

被引：49

作者：

Radhakrishnan, Prakash ^{[1
]}

Chachadi, Vishwanath ^{[1
]}

Lin, Ming-Fong ^{[1
,2
]}

Singh, Rakesh ^{[2
,3
]}

Kannagi, Reiji ^{[4
]}

Cheng, Pi-Wan ^{[1
,2
]}

机构：

[1] Univ Nebraska Med Ctr, Dept Biochem & Mol Biol, Coll Med, Omaha, NE 68198 USA

[2] Univ Nebraska Med Ctr, Eppley Canc Ctr Res Canc & Allied Dis, Omaha, NE 68198 USA

[3] Univ Nebraska Med Ctr, Dept Pathol & Microbiol, Omaha, NE 68198 USA

[4] Aichi Canc Ctr, Nagoya, Aichi 464, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 409卷 / 03期

关键词：

LNCaP cells; sLe(x); TNF alpha; Glycogenes; Motility and invasion; SIALYL-LEWIS-X; HIGH ENDOTHELIAL VENULES; TUMOR-NECROSIS-FACTOR; CARCINOMA MUCINS; BREAST-CANCER; P-SELECTIN; INCREASES; IDENTIFICATION; BIOSYNTHESIS; INFLAMMATION;

D O I：

10.1016/j.bbrc.2011.05.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sialyl Lewis x (sLe(x)) plays an important role in cancer metastasis. But, the mechanism for its production in metastatic cancers remains unclear. The objective of current study was to examine the effects of a pro-inflammatory cytokine on the expression of glycosyltransferase and sulfotransferase genes involved in the synthesis of selectin ligands in a prostate cancer cell line. Androgen-independent human lymph node-derived metastatic prostate cancer cells (C-81 LNCaP), which express functional androgen receptor and mimic the castration-resistant advanced prostate cancer, were used. TNF alpha treatment of these cells increased their binding to P-, E- and L-selectins, anti-sLe(x) antibody, and anti-6-sulfo-sialyl Lewis x antibody by 12%, 240%, 43%, 248% and 21%, respectively. Also, the expression of C2GnT-1, B4GalT1, Glc-NAc6ST3, and ST3Gal3 genes was significantly upregulated. Further treatment of TNF alpha-treated cells with either anti-sLe(x) antibody or E-selectin significantly suppressed their in vitro migration (81% and 52%, respectively) and invasion (45% and 56%, respectively). Our data indicate that TNF alpha treatment enhances the motility and invasion properties of LNCaP C-81 cells by increasing the formation of selectin ligands through stimulation of the expression of selective glycosyl- and sulfotransferase genes. These results support the hypothesis that inflammation contributes to cancer metastasis. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：436 / 441

页数：6

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