DNA copy number aberrations associated with the clinicopathological features of colorectal cancers: Identification of genomic biomarkers by array-based comparative genomic hybridization

被引：19
作者：
Nakao, Motonao [1 ]
Kawauchi, Shigeto [1 ]
Uchiyama, Tetsuji [2 ]
Adachi, Jun [2 ]
Ito, Hideaki [1 ]
Chochi, Yasuyo [1 ]
Furuya, Tomoko [1 ]
Oga, Atsunori [1 ]
Sasaki, Kohsuke [1 ]
机构：
[1] Yamaguchi Univ, Grad Sch Med, Dept Pathol, Yamaguchi 7558505, Japan
[2] Natl Hosp Org, Iwakuni Med Ctr, Dept Surg, Yamaguchi, Japan
来源：
ONCOLOGY REPORTS | 2011年 / 25卷 / 06期
关键词：
colorectal cancer; array-based comparative genome hybridization; DNA copy number aberration; genomic biomarker; JAG1;
D O I：
10.3892/or.2011.1246
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The aim of the present study was to investigate the chromosomal aberrations that are linked with the crucial clinicopathological features of colorectal cancer (CRC) and its prognosis by array-based comparative genomic hybridization (CGH). Fresh-frozen tumor tissues of 94 cases of CRC were analyzed by using bacterial artificial chromosome (BAC) CGH slides spotted with 4030 human BAC clones, which covered the whole range of the human genome at an average interval of 0.83 mega base pairs. DNA copy number aberrations (DCNAs) were identified in association with clinicopathological features: a gain of 8q24.3 and losses of 9q33.1 and 20p12.2 were associated with lymph node metastasis, gain of 8q24.3 and loss of 9q33.1 with disease stage, gain of 8q21.11 and loss of 10q21.3 with lymphovascular invasion and losses of 3p25.1, 10p15.3, 12q15 and 17p13.1 for venous invasion. These aberrations can be regarded as genomic biomarkers to predict the clinical outcome of patients with CRC, and are expected to serve to individualize the treatment of CRC patients.
引用
收藏
页码：1603 / 1611
页数：9
相关论文
共 16 条
[1]   A GENETIC MODEL FOR COLORECTAL TUMORIGENESIS [J].
FEARON, ER ;
VOGELSTEIN, B .
CELL, 1990, 61 (05) :759-767
[2]  
Hamilton S R., 2000, World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of the Digestive System, P105
[3]   Genetic instability in colorectal cancers [J].
Lengauer, C ;
Kinzler, KW ;
Vogelstein, B .
NATURE, 1997, 386 (6625) :623-627
[4]   Genetic instabilities in human cancers [J].
Lengauer, C ;
Kinzler, KW ;
Vogelstein, B .
NATURE, 1998, 396 (6712) :643-649
[5]  
Liu XP, 2007, ONCOL REP, V17, P261
[6]   Genetic association of CTNNA3 with late-onset Alzheimer's disease in females [J].
Miyashita, Akinori ;
Arai, Hiroyuki ;
Asada, Takashi ;
Imagawa, Masaki ;
Matsubara, Etsuro ;
Shoji, Mikio ;
Higuchi, Susumu ;
Urakami, Katsuya ;
Kakita, Akiyoshi ;
Takahashi, Hitoshi ;
Toyabe, Shinichi ;
Akazawa, Kohei ;
Kanazawa, Ichiro ;
Ihara, Yasuo ;
Kuwano, Ryozo .
HUMAN MOLECULAR GENETICS, 2007, 16 (23) :2854-2869
[7]   Identification of DNA copy number aberrations associated with metastases of colorectal cancer using array CGH profiles [J].
Nakao, Motonao ;
Kawauchi, Shigeto ;
Furuya, Tomoko ;
Uchiyama, Tetuji ;
Adachi, Jun ;
Okada, Takae ;
Ikemoto, Kenzo ;
Oga, Atsunori ;
Sasaki, Kohsuke .
CANCER GENETICS AND CYTOGENETICS, 2009, 188 (02) :70-76
[8]   High resolution genomic analysis of sporadic breast cancer using array-based comparative genomic hybridization [J].
Naylor, TL ;
Greshock, J ;
Wang, Y ;
Colligon, T ;
Yu, QC ;
Clemmer, V ;
Zaks, TZ ;
Weber, BL .
BREAST CANCER RESEARCH, 2005, 7 (06) :R1186-R1198
[9]   High resolution analysis of DNA copy number variation using comparative genomic hybridization to microarrays [J].
Pinkel, D ;
Seagraves, R ;
Sudar, D ;
Clark, S ;
Poole, I ;
Kowbel, D ;
Collins, C ;
Kuo, WL ;
Chen, C ;
Zhai, Y ;
Dairkee, SH ;
Ljung, BM ;
Gray, JW ;
Albertson, DG .
NATURE GENETICS, 1998, 20 (02) :207-211
[10]   High-level coexpression of JAG1 and NOTCH1 is observed in human breast cancer and is associated with poor overall survival [J].
Reedijk, M ;
Odorcic, S ;
Chang, L ;
Zhang, H ;
Miller, N ;
McCready, DR ;
Lockwood, G ;
Egan, SE .
CANCER RESEARCH, 2005, 65 (18) :8530-8537
12