A Poly(acrylic acid)-block-Poly(L-glutamic acid) Diblock Copolymer with Improved Cell Adhesion for Surface Modification

被引：19
作者：
Cao, Bin [2 ]
Yan, Shifeng [2 ]
Zhang, Kunxi [2 ]
Song, Zhijiang [2 ]
Cao, Tian [2 ]
Chen, Xuesi [1 ]
Cui, Lei [3 ]
Yin, Jingbo [2 ]
机构：
[1] Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Changchun 130022, Peoples R China
[2] Shanghai Univ, Dept Polymer Mat, Shanghai, Peoples R China
[3] Shanghai Jiao Tong Univ, Sch Med, Dept Plast & Reconstruct Surg, Shanghai Peoples Hosp 9, Shanghai 200011, Peoples R China
来源：
MACROMOLECULAR BIOSCIENCE | 2011年 / 11卷 / 07期
基金：
中国国家自然科学基金;
关键词：
adhesion; biological applications of polymers; films; layer growth; tissue engineering; HIGH-MOLECULAR-WEIGHT; ENDOTHELIAL-CELLS; STEM-CELLS; TISSUE; GROWTH; MULTILAYERS; DELIVERY; ADSORPTION; HYDROGELS; CHITOSAN;
D O I：
10.1002/mabi.201100010
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel PAA-b-PLGA diblock copolymer is synthesized and characterized that has excellent cell adhesion and biocompatibility. Fluorescent DiO labeling is used to monitor the attachment and growth of hASCs on the film surface, and cell proliferation over time is studied. Results show that PLLA modified by a CS/PAA-b-PLGA multilayer film can promote the attachment of human hASCs and provide an advantageous environment for their proliferation. The multilayer film presents excellent biocompatibility and cell adhesive properties, which will provide a new choice for improving the cell attachment in surface modification for tissue engineering. Hydroxyl, carboxyl and amine groups in the CS/PAA-b-PLGA multilayer film may be combined with drugs and growth factors for therapy and differentiation.
引用
收藏
页码：970 / 977
页数：8
相关论文
共 32 条
[1]   POLYPEPTIDES .3. THE SYNTHESIS OF HIGH MOLECULAR WEIGHT POLY-GAMMA-BENZYL-L-GLUTAMATES [J].
BLOUT, ER ;
KARLSON, RH .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1956, 78 (05) :941-946
[2]   Endothelial cells grown on thin polyelectrolyte mutlilayered films: an evaluation of a new versatile surface modification [J].
Boura, C ;
Menu, P ;
Payan, E ;
Picart, C ;
Voegel, JC ;
Muller, S ;
Stoltz, JF .
BIOMATERIALS, 2003, 24 (20) :3521-3530
[3]  
Cao T, 2006, CHEM J CHINESE U, V27, P369
[4]   Competitive adsorption of uncharged macromolecules from homopolymer-copolymer mixtures [J].
Csaki, Katalin F. ;
Csempesz, Ferenc .
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2007, 299 (1-3) :217-223
[5]   Repair of cranial bone defects with adipose derived stem cells and coral scaffold in a canine model [J].
Cui, Lei ;
Liu, Bo ;
Liu, Guangpeng ;
Zhang, Wenjie ;
Cen, Lian ;
Sun, Jian ;
Yin, Shuo ;
Liu, Wei ;
Cao, Yilin .
BIOMATERIALS, 2007, 28 (36) :5477-5486
[6]   Expanded adipose-derived stem cells suppress mixed lymphocyte reaction by secretion of prostaglandin E2 [J].
Cui, Lei ;
Yin, Shuo ;
Liu, Wei ;
Li, Ningli ;
Zhang, Wenjie ;
Cao, Yilin .
TISSUE ENGINEERING, 2007, 13 (06) :1185-1195
[7]   Composites of plasma treated poly(etherimide) films with gold nanoparticles and lysine through layer by layer assembly: a "friendly-rough" surface for cell adhesion and proliferation for tissue engineering applications [J].
D'Britto, V. ;
Tiwari, S. ;
Purohit, V. ;
Wadgaonkar, P. P. ;
Bhoraskar, S. V. ;
Bhonde, R. R. ;
Prasad, B. L. V. .
JOURNAL OF MATERIALS CHEMISTRY, 2009, 19 (04) :544-550
[8]   THE PREPARATION OF N-CARBOXYANHYDRIDES OF ALPHA-AMINO-ACIDS USING BIS(TRICHLOROMETHYL)CARBONATE [J].
DALY, WH ;
POCHE, D .
TETRAHEDRON LETTERS, 1988, 29 (46) :5859-5862
[9]   Synthesis of a novel structural triblock copolymer of poly(γ-benzyl-L-glutamic acid)-b-poly(ethylene oxide)-b-poly(ε-caprolactone) [J].
Deng, MX ;
Wang, R ;
Rong, GZ ;
Sun, JR ;
Zhang, XF ;
Chen, XS ;
Jing, XB .
BIOMATERIALS, 2004, 25 (17) :3553-3558
[10]   Bone formation mediated by synergy-acting growth factors embedded in a polyelectrolyte multilayer film [J].
Dierich, Andree ;
Le Guen, Erell ;
Messaddeq, Nadia ;
Stoltz, Jean-Francois ;
Netter, Patrick ;
Schaaf, Pierre ;
Voegel, Jean-Claude ;
Benkirane-Jessel, Nadia .
ADVANCED MATERIALS, 2007, 19 (05) :693-+
1234