Antiviral activity and mode of action of a peptide isolated from Sorghum bicolor

In this paper, we describe the purification of an antiviral peptide from seeds of Sorghum bicolor L. by a procedure that included gel filtration, ion exchange, and high-performance liquid chromatography (HPLC) in a reversed-phase column. Its molecular weight, determined by chromatographic mobility on the Shim-pack DIOL-150 gel permeation column in HPLC, was found to be 2000 Da. The peptide designated 2 kD peptide strongly inhibited the replication of herpes simplex virus type I (HSV-1), dose-dependently, at 40-90% of the control level, after incubation with 10-50 mu M of the peptide, with EC50 and EC90 values of 6.25 and 15.25 mu M, respectively. The IC50 value of the 2 kD peptide against Vero cells was 250 mu M. Pre-incubation of HSV-1 with various concentrations of the 2 kD peptide showed dose-dependent cytopathic effects (CPE) reduction patterns at concentrations from 6.25 to 50 mu M. The presence of the 2 kD peptide before HSV-1 infections showed moderate inhibition of virus-induced CPE as compared to during or after infections, with EC50 values of 12.5, 6.25, and 6.25 mu M, respectively. Similar results were observed when the 2 kD peptide was assayed against bovine herpes virus (BHV), an enveloped virus like HSV-1. On the other hand, the 2 kD peptide showed weak activity against poliovirus type 1, a non-enveloped virus. Taken together, these results indicate that the 2 kD peptide was able not only to inhibit the initiation and the spread of infection, but also had an in vitro prophylactic effect against HSV-1 infection. (c) 2007 Elsevier GmbH. All rights reserved.