Immobilization of Pholiota adiposa xylanase onto SiO2 nanoparticles and its application for production of xylooligosaccharides

被引：21

作者：

Dhiman, Saurabh Sudha ^{[1
,2
]}

Jagtap, Sujit Sadashiv ^{[1
]}

Jeya, Marimuthu ^{[1
,2
]}

Haw, Jung-Rim ^{[2
]}

Kang, Yun Chan ^{[1
]}

Lee, Jung-Kul ^{[1
,2
]}

机构：

[1] Konkuk Univ, Dept Chem Engn, Seoul 143701, South Korea

[2] Konkuk Univ, Inst SK KU Biomat, Seoul 143701, South Korea

来源：

BIOTECHNOLOGY LETTERS | 2012年 / 34卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

Immobilization; Response surface methodology; SiO2; Xylanase; Xylooligosaccharide; PURIFICATION;

D O I：

10.1007/s10529-012-0902-y

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Enhanced yields of different lignocellulases were obtained under statistically-optimized parameters using Pholiota adiposa. The k (cat) value (4,261 s(-1)) of purified xylanase under standard assay conditions was the highest value ever reported. On covalent immobilization of the crude xylanase preparation onto functionalized silicon oxide nanoparticles, 66 % of the loaded enzyme was retained on the particle. Immobilized enzyme gave 45 % higher concentrations of xylooligosaccharides compared to the free enzyme. After 17 cycles, the immobilized enzyme retained 97 % of the original activity, demonstrating its prospects for the synthesis of xylooligosaccharides in industrial applications.

引用

页码：1307 / 1313

页数：7

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