Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)

被引：5

作者：

Naskar, Soumen ^{[1
]}

Deb, Sitangsu M. ^{[1
]}

Niranjan, Saket K. ^{[1
]}

Kumar, Subodh ^{[1
]}

Sharma, Deepak ^{[1
]}

Sakaram, Durgam ^{[1
]}

Sharma, Arjava ^{[1
]}

机构：

[1] Indian Vet Res Inst, Div Anim Genet, Izatnagar 243122, Uttar Pradesh, India

来源：

GENETICS AND MOLECULAR BIOLOGY | 2012年 / 35卷 / 01期

关键词：

water buffalo; cDNA; DRB; MHC; phylogeny; CATTLE;

D O I：

10.1590/S1415-47572012005000013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the beta(1) domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A.

引用

页码：95 / 98

页数：4

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