Additive effect of tumor necrosis factor-α and endotoxin on bilirubin cytotoxicity

被引：8
|
作者：
Ngai, KC [1 ]
Yeung, CY [1 ]
机构：
[1] Univ Hong Kong, Queen Mary Hosp, Dept Paediat, Hong Kong, Peoples R China
来源：
PEDIATRIC RESEARCH | 1999年 / 45卷 / 04期
关键词：
D O I：
10.1203/00006450-199904010-00012
中图分类号：
R72 [儿科学];
学科分类号：
100202 ;
摘要：
Clinical observations suggest that sepsis may enhance the risk of kernicterus. This study investigated the combined effects of bilirubin, endotoxin, and tumor necrosis factor-alpha (TNF-alpha), which simulate sepsis in a jaundiced mouse fibroblast cell line. The horseradish peroxidase oxidation method was applied for bilirubin-albumin titration studies to lest the effect of endotoxin and TNF-alpha on bilirubin-albumin binding. A modified 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide method was used to study cytotoxicity. Bilirubin caused cytotoxicity in a dose-dependent manner in the cultured mouse fibroblasts. Such an effect was significantly amplified by TNF-alpha and endotoxin. TNF-alpha and endotoxin had no effect on the bilirubin-albumin titration curves. Our results have shown that TNF-alpha and endotoxin increase the cytotoxicity of bilirubin. These findings provide supportive evidence that sepsis would increase the risk of tissue damage by bilirubin.
引用
收藏
页码：526 / 530
页数：5
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