共 25 条
Development of liquid phase microextraction based on manual shaking and ultrasound-assisted emulsification method for analysis of organochlorine pesticides in aqueous samples
被引:49
作者:
Wei, Shuo-Yang
[1
]
Leong, Mei-I
[1
]
Li, Yee
[1
]
Huang, Shang-Da
[1
]
机构:
[1] Natl Tsing Hua Univ, Dept Chem, Hsinchu 30013, Taiwan
关键词:
Manual shaking;
Ultrasound-assisted emulsification;
Dispersive liquid-liquid microextraction;
Organochlorine pesticides;
Gas chromatography;
Improved solvent collection system;
CHROMATOGRAPHY-MASS SPECTROMETRY;
GAS-CHROMATOGRAPHY;
WATER SAMPLES;
ORGANIC-SOLVENT;
SINGLE DROP;
D O I:
10.1016/j.chroma.2011.10.084
中图分类号:
Q5 [生物化学];
学科分类号:
071010 ;
081704 ;
摘要:
A novel method using sample preparation method, "ultrasound-assisted emulsification microextraction" (USAEME) with manual shaking, coupled with gas chromatography using and an electron capture detector (GC-ECD) was developed for the analysis of organochlorine pesticides (OCPs) in aqueous samples. The apparatus is simple and easy to operate. After manual shaking for 10 s, ultrasound was used to accelerate emulsification of the organic solvent (1-decanol) in aqueous solution. Only 10 mu L of the low-toxicity extraction solvent is used in this method; no dispersive solvent is required and the total extraction time is similar to 4 Mill. Manual shaking before ultrasound-assisted emulsification enhances the extraction efficiency by >100%. The effects of horizontal and vertical orientation as well as the location of the sample within the ultrasonic bath were studied. After centrifugation, we used an improved solvent collection system (ISCS) to reduce the amount of extraction solvent required. A 1 mu L sample of the extract was injected into the GC column. Under optimum conditions, the linear range of the method is 5-2500 ng L(-1) for most of the OCPs, and the limit of detection of the method ranged from 0.6 to 2.9 ng L(-1) The relative recoveries ranged from 75 to 107% for sea water and from 70 to 99% for field fresh water. The method, which provides good enrichment factors, low LODs and minimization of the consumption of organic solvent. provides a rapid, simple and environment-friendly procedure for determining OCPs in aqueous samples. (C) 2011 Elsevier B.V. All rights reserved.
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页码:9142 / 9148
页数:7
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