Glucoamylase production from food waste by Aspergillus niger under submerged fermentation

被引：66
作者：
Wang, Qunhui [1 ,2 ]
Wang, Xiaoqiang [1 ]
Wang, Xuming [3 ]
Ma, Hongzhi [1 ]
机构：
[1] Harbin Inst Technol, Dept Environm Sci & Engn, Harbin 150090, Peoples R China
[2] Univ Sci & Technol Beijing, Dept Environm Engn, Beijing 100083, Peoples R China
[3] Jilin Univ, Coll Plant Sci, Changchun 130062, Peoples R China
来源：
PROCESS BIOCHEMISTRY | 2008年 / 43卷 / 03期
基金：
中国国家自然科学基金;
关键词：
glucoamylase; food waste; Aspergillus niger; submerged fermentation; smash process; enzymatic hydrolysis;
D O I：
10.1016/j.procbio.2007.12.010
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The potential of food waste (FW) used as substrate for the glucoamylase production by Aspergillus niger UV-60 under submerged fermentation was investigated. Results of Plackett-Burman experimental design suggested nutrients including yeast extract, (NH4)(2)SO4, KH2PO4, MgSO4 center dot 7H(2)O, FeSO4 center dot 7H(2)O and CaCl2 had no significant influence on the glucoamylase production. At the optimum concentration of 2.50% (dry basis), smashed food waste (smashed-FW) produced glucoamylase of 126 U/mI after 96 h fermentation, whereas 137 U/ml of glucoamylase could be attained within the same time from raw food waste (raw-FW) of 3.75%. The particle size showed little effect during enzyme production, and could be degraded easily, thus the smash process need not be adopted in this study. The hydrolysis experiment with the produced enzyme demonstrated that about 60 g/l of reducing sugar could be attained from 10% smashed-FW after 2-h hydrolysis. The utilization of food waste for glucoamylase production could save the cost as well as reduce the pollution threatens. (C) 2008 Elsevier Ltd. All rights reserved.
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收藏
页码：280 / 286
页数：7
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