Cantharidin suppresses gastric cancer cell migration/invasion by inhibiting the PI3K/Akt signaling pathway via CCAT1

被引：51

作者：

Song, Mengyun ^{[1
,2
]}

Wang, Xianfei ^{[3
]}

Luo, Yajun ^{[3
]}

Liu, Zilin ^{[3
]}

Tan, Wang ^{[3
]}

Ye, Pengcheng ^{[3
]}

Fu, Zhiming ^{[6
]}

Lu, Fei ^{[3
]}

Xiang, Wanping ^{[3
]}

Tang, Linghan ^{[3
]}

Yao, Lin ^{[3
]}

Nie, Yuqiang ^{[5
]}

Xiao, Jiangwei ^{[4
]}

机构：

[1] Chengdu Med Coll, Dept Gastroenterol, Affiliated Hosp 1, Chengdu, Sichuan, Peoples R China

[2] Nankai Univ, Sch Med, Tianjin, Peoples R China

[3] North Sichuan Med Coll, Dept Gastroenterol, Affiliated Hosp, Nanchong, Sichuan, Peoples R China

[4] Chengdu Med Coll, Affiliated Hosp 1, Dept Gastrointestinal Surg, 278 Baoguang Rd, Chengdu, Sichuan, Peoples R China

[5] South China Univ Technol, Affiliated Hosp 2, Guangzhou Peoples Hosp 1, Dept Gastroenterol,Guangzhou Digest Dis Ctr, Guangzhou, Peoples R China

[6] Hainan Gen Hosp, Dept Gastrointestinal Surg 1, Haikou, Hainan, Peoples R China

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 2020年 / 317卷

基金：

中国国家自然科学基金;

关键词：

Long non-coding RNA; Gastric cancer; CCAT1; Cantharidin; PI3K/AKt; APOPTOSIS; PROMOTES; INVASION; GROWTH;

D O I：

10.1016/j.cbi.2020.108939

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cantharidin (CTD) is a traditional Chinese medicine that shows an anticancer effects in multiple types of cancer cells. However, the mechanism of CTD anti-cancer function in gastric cancer (GC) is still unclear. The aim of the present study was to investigate the underlying mechanism that CTD inhibits proliferation and migration through suppression of the PI3K/Akt signaling. CTD induced GC cell apoptosis and inhibited metastasis measured by CCK8 assays as well as wound healing assays and transwell assays. Mechanistic investigations suggested that CTD modulated the PI3K/Akt signaling via western-blot and quantitative q-PCR. In addition, we identified and confirmed CCAT1 as a novel direct target of CTD inhibited PI3K/AKt signaling expression. In conclusion, our results provide new point into the critical role of CTD in suppressing PI3K/Akt signaling via down-regulation of CCAT1, resulting in suppression GC cell growth and migration/invasion.

引用

页数：11

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