Enterocin P selectively dissipates the membrane potential of Enterococcus faecium T136

被引：61

作者：

Herranz, C

Chen, Y

Chung, HJ

Cintas, LM

Hernández, PE

Montville, TJ

Chikindas, ML

机构：

[1] Rutgers State Univ, Dept Food Sci, New Brunswick, NJ 08901 USA

[2] Univ Complutense Madrid, Fac Vet, Dept Nutr & Bromatol 3, E-28040 Madrid, Spain

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 04期

关键词：

D O I：

10.1128/AEM.67.4.1689-1692.2001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Enterocin P is a pediocin-like, broad-spectrum bacteriocin which displays a strong inhibitory activity against Listeria monocytogenes. The bacteriocin was purified from the culture supernatant of Enterococcus faecium P13, and its molecular mechanism of action against the sensitive strain E. faecium T136 was evaluated. Although enterocin P caused significant reduction of the membrane potential (Delta Psi) and the intracellular ATP pool of the indicator organism, the pH gradient (Delta pH) component of the proton motive force (Deltap) was not dissipated. By contrast, enterocin P caused carboxyfluorescein efflux from E. faecium T136-derived liposomes.

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页码：1689 / 1692

页数：4

相关论文

共 35 条

[1] KINETIC-STUDIES OF THE ACTION OF LACTACIN-F, A BACTERIOCIN PRODUCED BY LACTOBACILLUS-JOHNSONII THAT FORMS PORATION COMPLEXES IN THE CYTOPLASMIC MEMBRANE [J].

ABEE, T ;

KLAENHAMMER, TR ;

LETELLIER, L .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (03) :1006-1013

[2] Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins [J].

Aymerich, T ;

Holo, H ;

Havarstein, LS ;

Hugas, M ;

Garriga, M ;

Nes, IF .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (05) :1676-1682

[3] Interactions of nisin and pediocin PA-1 with closely related lactic acid bacteria that manifest over 100-fold differences in bacteriocin sensitivity [J].

Bennik, MHJ ;

Verheul, A ;

Abee, T ;

NaaktgeborenStoffels, G ;

Gorris, LGM ;

Smid, EJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (09) :3628-3636

[4] A novel bacteriocin with a YGNGV motif from vegetable-associated Enterococcus mundtii:: full characterization and interaction with target organisms [J].

Bennik, MHJ ;

Vanloo, B ;

Brasseur, R ;

Gorris, LGM ;

Smid, EJ .

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1998, 1373 (01) :47-58

[5]

BRUNOI MEC, 1992, APPL ENVIRON MICROB, V59, P3003

[6] Enterocin B, a new bacteriocin from Enterococcus faecium T136 which can act synergistically with enterocin A [J].

Casaus, P ;

Nilsen, T ;

Cintas, LM ;

Nes, IF ;

Hernandez, PE ;

Holo, H .

MICROBIOLOGY-SGM, 1997, 143 :2287-2294

[7] EFFLUX OF IONS AND ATP DEPLETION INDUCED BY PEDIOCIN PA-1 ARE CONCOMITANT WITH CELL-DEATH IN LISTERIA-MONOCYTOGENES SCOTT-A [J].

CHEN, Y ;

MONTVILLE, TJ .

JOURNAL OF APPLIED BACTERIOLOGY, 1995, 79 (06) :684-690

[8]

CHEN Y, 1997, APPL ENVIRON MICROB, V6, P4770

[9] Functional characterization of pediocin PA-1 binding to liposomes in the absence of a protein receptor and its relationship to a predicted tertiary structure [J].

Chen, YH ;

Shapira, R ;

Eisenstein, M ;

Montville, TJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (02) :524-531

[10] PEDIOCIN PA-1, A BACTERIOCIN FROM PEDIOCOCCUS-ACIDILACTICI PAC1.0, FORMS HYDROPHILIC PORES IN THE CYTOPLASMIC MEMBRANE OF TARGET-CELLS [J].

CHIKINDAS, ML ;

GARCIAGARCERA, MJ ;

DRIESSEN, AJM ;

LEDEBOER, AM ;

NISSENMEYER, J ;

NES, IF ;

ABEE, T ;

KONINGS, WN ;

VENEMA, G .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (11) :3577-3584

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