Rapid simultaneous lipid extraction and transesterification for fatty acid analyses

被引：306

作者：

Rodriguez-Ruiz, J ^{[1
]}

Belarbi, EH

Sanchez, JLG

Alonso, DL

机构：

[1] Univ Almeria, Dept Biol Aplicada, Almeria 04071, Spain

[2] Univ Almeria, Dept Ingn Quim, Almeria 04071, Spain

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 09期

关键词：

fatty acid; GLC; fatty acid methyl esters; transesterification;

D O I：

10.1023/A:1008812904017

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved adaptation of the direct transesterification method of Lepage and Roy (J. Lipid Res. 25, 1391-96, 1984) for the preparation of fatty acid methyl esters allows notable saving of time and reagents. The material being analysed is heated for 10 minutes with methanol, acetyl chloride and hexane.

引用

页码：689 / 691

页数：3

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