Rapid simultaneous lipid extraction and transesterification for fatty acid analyses

被引：302
作者：
Rodriguez-Ruiz, J [1 ]
Belarbi, EH
Sanchez, JLG
Alonso, DL
机构：
[1] Univ Almeria, Dept Biol Aplicada, Almeria 04071, Spain
[2] Univ Almeria, Dept Ingn Quim, Almeria 04071, Spain
来源：
BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 09期
关键词：
fatty acid; GLC; fatty acid methyl esters; transesterification;
D O I：
10.1023/A:1008812904017
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An improved adaptation of the direct transesterification method of Lepage and Roy (J. Lipid Res. 25, 1391-96, 1984) for the preparation of fatty acid methyl esters allows notable saving of time and reagents. The material being analysed is heated for 10 minutes with methanol, acetyl chloride and hexane.
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页码：689 / 691
页数：3
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