Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer

被引：101

作者：

Cao, Liwei ^{[1
,2
,3
,4
]}

Pauthner, Matthias ^{[2
,3
,4
]}

Andrabi, Raiees ^{[2
,3
,4
]}

Rantalainen, Kimmo ^{[3
,4
,5
]}

Berndsen, Zachary ^{[3
,4
,5
]}

Diedrich, Jolene K. ^{[1
]}

Menis, Sergey ^{[2
,3
,4
]}

Sok, Devin ^{[2
,3
,4
]}

Bastidas, Raiza ^{[2
,3
,4
]}

Park, Sung-Kyu Robin ^{[1
]}

Delahunty, Claire M. ^{[1
]}

He, Lin ^{[1
]}

Guenaga, Javier ^{[2
,3
,4
]}

Wyatt, Richard T. ^{[2
,3
,4
]}

Schief, William R. ^{[2
,3
,4
]}

Ward, Andrew B. ^{[3
,4
,5
]}

Yates, John R., III ^{[1
]}

Burton, Dennis R. ^{[2
,3
,4
,6
]}

Paulson, James C. ^{[1
,2
,3
,4
]}

机构：

[1] Scripps Res Inst, Dept Mol Med, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Dept Immunol & Microbiol, La Jolla, CA 92037 USA

[3] Scripps Res Inst, Ctr HIV AIDS Vaccine Immunol & Immunogen Discover, La Jolla, CA 92037 USA

[4] Scripps Res Inst, IAVI Neutralizing Antibody Ctr, La Jolla, CA 92037 USA

[5] Scripps Res Inst, Dept Integrat Struct & Computat Biol, La Jolla, CA 92037 USA

[6] Ragon Inst MGH MIT & Harvard, Cambridge, MA USA

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

关键词：

BROADLY NEUTRALIZING ANTIBODIES; SITE-SPECIFIC ANALYSIS; DEPENDENT EPITOPE; GLYCOPROTEIN COMPLEX; ENV; PROTEIN; GLYCOSYLATION; SPECIFICITY; MODEL; IDENTIFICATION;

D O I：

10.1038/s41467-018-06121-4

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

As the sole target of broadly neutralizing antibodies (bnAbs) to HIV, the envelope glycoprotein (Env) trimer is the focus of vaccination strategies designed to elicit protective bnAbs in humans. Because HIV Env is densely glycosylated with 75-90 N-glycans per trimer, most bnAbs use or accommodate them in their binding epitope, making the glycosylation of recombinant Env a key aspect of HIV vaccine design. Upon analysis of three HIV strains, we here find that site-specific glycosylation of Env from infectious virus closely matches Envs from corresponding recombinant membrane-bound trimers. However, viral Envs differ significantly from recombinant soluble, cleaved (SOSIP) Env trimers, strongly impacting antigenicity. These results provide a benchmark for virus Env glycosylation needed for the design of soluble Env trimers as part of an overall HIV vaccine strategy.

引用

页数：14

共 68 条

[51] UCSF chimera - A visualization system for exploratory research and analysis
Pettersen, EF
Goddard, TD
Huang, CC
Couch, GS
Greenblatt, DM
Meng, EC
Ferrin, TE
[J]. JOURNAL OF COMPUTATIONAL CHEMISTRY, 2004, 25 (13) : 1605 - 1612
[52] Cell- and Protein-Directed Glycosylation of Native Cleaved HIV-1 Envelope
Pritchard, Laura K.
Harvey, David J.
Bonomelli, Camille
Crispin, Max
Doores, Katie J.
[J]. JOURNAL OF VIROLOGY, 2015, 89 (17) : 8932 - 8944
[53] Structural Constraints Determine the Glycosylation of HIV-1 Envelope Trimers
Pritchard, Laura K.
Vasiljevic, Snezana
Ozorowski, Gabriel
Seabright, Gemma E.
Cupo, Albert
Ringe, Rajesh
Kim, Helen J.
Sanders, Rogier W.
Doores, Katie J.
Burton, Dennis R.
Wilson, Ian A.
Ward, Andrew B.
Moore, John P.
Crispin, Max
[J]. CELL REPORTS, 2015, 11 (10): : 1604 - 1613
[54] A Native-Like SOSIP.664 Trimer Based on an HIV-1 Subtype B env Gene
Pugach, Pavel
Ozorowski, Gabriel
Cupo, Albert
Ringe, Rajesh
Yasmeen, Anila
de Val, Natalia
Derking, Ronald
Kim, Helen J.
Korzun, Jacob
Golabek, Michael
de los Reyes, Kevin
Ketas, Thomas J.
Julien, Jean-Philippe
Burton, Dennis R.
Wilson, Ian A.
Sanders, Rogier W.
Klasse, P. J.
Ward, Andrew B.
Moore, John P.
[J]. JOURNAL OF VIROLOGY, 2015, 89 (06) : 3380 - 3395
[55] A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies
Sanders, Rogier W.
Derking, Ronald
Cupo, Albert
Julien, Jean-Philippe
Yasmeen, Anila
de Val, Natalia
Kim, Helen J.
Blattner, Claudia
de la Pena, Alba Torrents
Korzun, Jacob
Golabek, Michael
de los Reyes, Kevin
Ketas, Thomas J.
van Gils, Marit J.
King, C. Richter
Wilson, Ian A.
Ward, Andrew B.
Klasse, P. J.
Moore, John P.
[J]. PLOS PATHOGENS, 2013, 9 (09)
[56] Stabilization of the soluble, cleaved, trimeric form of the envelope glycoprotein complex of human immunodeficiency virus type 1
Sanders, RW
Vesanen, M
Schuelke, N
Master, A
Schiffner, L
Kalyanaraman, R
Paluch, M
Berkhout, B
Maddon, PJ
Olson, WC
Lu, M
Moore, JP
[J]. JOURNAL OF VIROLOGY, 2002, 76 (17) : 8875 - 8889
[57] The mannose-dependent epitope for neutralizing antibody 2G12 on human immunodeficiency virus type 1 glycoprotein gp120
Sanders, RW
Venturi, M
Schiffner, L
Kalyanaraman, R
Katinger, H
Lloyd, KO
Kwong, PD
Moore, JP
[J]. JOURNAL OF VIROLOGY, 2002, 76 (14) : 7293 - 7305
[58] A Prominent Site of Antibody Vulnerability on HIV Envelope Incorporates a Motif Associated with CCR5 Binding and Its Camouflaging Glycans
Sok, Devin
Pauthner, Matthias
Briney, Bryan
Lee, Jeong Hyun
Saye-Francisco, Karen L.
Hsueh, Jessica
Ramos, Alejandra
Le, Khoa M.
Jones, Meaghan
Jardine, Joseph G.
Bastidas, Raiza
Sarkar, Anita
Liang, Chi-Hui
Shivatare, Sachin S.
Wu, Chung-Yi
Schief, William R.
Wong, Chi-Huey
Wilson, Ian A.
Ward, Andrew B.
Zhu, Jiang
Poignard, Pascal
Burton, Dennis R.
[J]. IMMUNITY, 2016, 45 (01) : 31 - 45
[59] Stavenhagen K, 2013, J MASS SPECTROM, V48, P627, DOI [10.1002/jms.3210, 10.1002/jms.3189]
[60] l Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G
Stewart-Jones, Guillaume B. E.
Soto, Cinque
Lemmin, Thomas
Chuang, Gwo-Yu
Druz, Aliaksandr
Kong, Rui
Thomas, Paul V.
Wagh, Kshitij
Zhou, Tongqing
Behrens, Anna-Janina
Bylund, Tatsiana
Choi, Chang W.
Davison, Jack R.
Georgiev, Ivelin S.
Joyce, M. Gordon
Do Kwon, Young
Pancera, Marie
Taft, Justin
Yang, Yongping
Zhang, Baoshan
Shivatare, Sachin S.
Shivatare, Vidya S.
Lee, Chang-Chun D.
Wu, Chung-Yi
Bewley, Carole A.
Burton, Dennis R.
Koff, Wayne C.
Connors, Mark
Crispin, Max
Baxa, Ulrich
Korber, Bette T.
Wong, Chi-Huey
Mascola, John R.
Kwong, Peter D.
[J]. CELL, 2016, 165 (04) : 813 - 826

← 1 2 3 4 5 6 7 →