Super-resolution imaging in thick scattering samples by structured illumination microscopy with dual nonlinear effects.

被引：1

作者：

Jiang, Shurong ^{[1
]}

Wang, Yanhui ^{[1
]}

Liu, Fan ^{[1
]}

Li, Qingru ^{[1
]}

Zhang, Han ^{[1
]}

机构：

[1] Sichuan Univ, Coll Elect Engn & Informat Technol, Chengdu, Sichuan, Peoples R China

来源：

FIFTH CONFERENCE ON FRONTIERS IN OPTICAL IMAGING TECHNOLOGY AND APPLICATIONS (FOI 2018) | 2018年 / 10832卷

基金：

美国国家科学基金会;

关键词：

Super-resolution; Fluorescence microscopy; Image processing; Patterned illumination; Stimulated emission depletion; Two-photon excitation; structured illumination microscopy; STIMULATED-EMISSION-DEPLETION; 2-PHOTON EXCITATION; RESOLUTION LIMIT; FLUORESCENCE; IMPROVES;

D O I：

10.1117/12.2506679

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

High-resolution, real-time and three-dimensional imaging in thick scattering specimens is of great significance in biology, yet meeting these requirements at the same time is fraught with challenges. In this work, we describe a method that combines structured illumination microscopy (SIM) with dual nonlinear effects, two-photon excitation (2PE) technique and stimulated emission depletion (STED), to further improve the imaging resolution in optical-thick samples relative to SIM. Utilizing a line-scanning geometry shaped by cylindrical lens to form structured illumination pattern, the imaging speed is greatly improved. Theoretical study and simulations are both performed to demonstrate the capability of this method to enhance resolution laterally and the potential for applications in real-time imaging for living tissue.

引用

页数：6

共 19 条

[1] Imaging intracellular fluorescent proteins at nanometer resolution [J].

Betzig, Eric ;

Patterson, George H. ;

Sougrat, Rachid ;

Lindwasser, O. Wolf ;

Olenych, Scott ;

Bonifacino, Juan S. ;

Davidson, Michael W. ;

Lippincott-Schwartz, Jennifer ;

Hess, Harald F. .

SCIENCE, 2006, 313 (5793) :1642-1645

[2] Single-wavelength two-photon excitation-stimulated emission depletion (SW2PE-STED) superresolution imaging [J].

Bianchini, Paolo ;

Harke, Benjamin ;

Galiani, Silvia ;

Vicidomini, Giuseppe ;

Diaspro, Alberto .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (17) :6390-6393

[3] 2-PHOTON LASER SCANNING FLUORESCENCE MICROSCOPY [J].

DENK, W ;

STRICKLER, JH ;

WEBB, WW .

SCIENCE, 1990, 248 (4951) :73-76

[4] Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy [J].

Gustafsson, MGL .

JOURNAL OF MICROSCOPY, 2000, 198 (02) :82-87

[5] Nonlinear structured-illumination microscopy: Wide-field fluorescence imaging with theoretically unlimited resolution [J].

Gustafsson, MGL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (37) :13081-13086

[6] Saturated patterned excitation microscopy - a concept for optical resolution improvement [J].

Heintzmann, R ;

Jovin, TM ;

Cremer, C .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 2002, 19 (08) :1599-1609

[7] BREAKING THE DIFFRACTION RESOLUTION LIMIT BY STIMULATED-EMISSION - STIMULATED-EMISSION-DEPLETION FLUORESCENCE MICROSCOPY [J].

HELL, SW ;

WICHMANN, J .

OPTICS LETTERS, 1994, 19 (11) :780-782

[8] Deep tissue two-photon microscopy [J].

Helmchen, F ;

Denk, W .

NATURE METHODS, 2005, 2 (12) :932-940

[9] Ultra-high resolution imaging by fluorescence photoactivation localization microscopy [J].

Hess, Samuel T. ;

Girirajan, Thanu P. K. ;

Mason, Michael D. .

BIOPHYSICAL JOURNAL, 2006, 91 (11) :4258-4272

[10] Super-Resolution Fluorescence Microscopy [J].

Huang, Bo ;

Bates, Mark ;

Zhuang, Xiaowei .

ANNUAL REVIEW OF BIOCHEMISTRY, 2009, 78 :993-1016

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