Identification of microcystins from three collection strains of Microcystis aeruginosa

被引：54

作者：

del Campo, Francisca F. ^{[1
]}

Ouahid, Youness ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Dept Biol, E-28049 Madrid, Spain

来源：

ENVIRONMENTAL POLLUTION | 2010年 / 158卷 / 09期

关键词：

LC-MS/MS; MALDI-TOF/MS; New microcystins; UTEX collection; UAM collection; CYCLIC HEPTAPEPTIDE HEPATOTOXINS; IONIZATION MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; CYANOBACTERIAL TOXINS; OSCILLATORIA-AGARDHII; BLOOM; DIVERSITY; DECOMPOSITION; COMMUNITIES; WIDESPREAD;

D O I：

10.1016/j.envpol.2010.06.018

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Microcystins (MCs) are toxic cyclic heptapeptides produced by various cyanobacteria genera, especially Microcystis. We identified 10 out of 12 MCs produced by three Microcystis aeruginosa strains from cyanobacteria collections, UTEX 2666, UTEX 2670 and UAM 1303, by using two analytical methods: Matrix-assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF/MS) and HPLC Photodiode Array Detector coupled to a hybrid Quadrupole Time of Flight Mass Spectrometry (HPLC-PDA-QTOF/MS). MALDI-TOF/MS failed to detect non-polar MCs, such as MC-LY and MC-LW. HPLC-QTOF/MS permitted the accurate identification of most MCs present in methanolic extracts. Besides, three new MCs, namely: [D-Glu(OCH3)(6), D-Asp(3)] MC-LAba, MC-YL and MC-YM were detected by HPLC-QTOF/MS. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：2906 / 2914

页数：9

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