Evaluation and Comparison of the Pathogenicity and Host Immune Responses Induced by a G2b Taiwan Porcine Epidemic Diarrhea Virus (Strain Pintung 52) and Its Highly Cell-Culture Passaged Strain in Conventional 5-Week-Old Pigs

被引：36

作者：

Chang, Yen-Chen ^{[1
]}

Kao, Chi-Fei ^{[1
]}

Chang, Chia-Yu ^{[1
]}

Jeng, Chian-Ren ^{[1
,2
]}

Tsai, Pei-Shiue ^{[2
]}

Pang, Victor Fei ^{[1
,2
]}

Chiou, Hue-Ying ^{[3
]}

Peng, Ju-Yi ^{[1
]}

Cheng, Ivan-Chen ^{[1
]}

Chang, Hui-Wen ^{[1
,2
]}

机构：

[1] Natl Taiwan Univ, Grad Inst Mol & Comparat Pathobiol, Sch Vet Med, 1,Sect 4,Roosevelt Rd, Taipei 10617, Taiwan

[2] Natl Taiwan Univ, Sch Vet Med, 1,Sect 4,Roosevelt Rd, Taipei 10617, Taiwan

[3] Natl Chung Hsing Univ, Grad Inst Vet Pathobiol, Coll Vet Med, 250 Kuo Kuang Rd, Taichung 402, Taiwan

来源：

VIRUSES-BASEL | 2017年 / 9卷 / 05期

关键词：

cell culture-adapted virus; conventional pig; neutralizing antibody; porcine epidemic diarrhea virus; viral shedding; ANTIBODY-RESPONSE; NURSING PIGLETS; PEDV; PROTECTION; VIRULENT; PATHOGENESIS; INOCULATION; INFECTION;

D O I：

10.3390/v9050121

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A genogroup 2b (G2b) porcine epidemic diarrhea virus (PEDV) Taiwan Pintung 52 (PEDVPT) strain was isolated in 2014. The pathogenicity and host antibody responses elicited by low-passage (passage 5; PEDVPT-P5) and high-passage (passage 96; PEDVPT-P96) PEDVPT strains were compared in post-weaning PEDV-seronegative pigs by oral inoculation. PEDVPT-P5-inoculation induced typical diarrhea during 1-9 days post inoculation with fecal viral shedding persisting for 26 days. Compared to PEDVPT-P5, PEDVPT-P96 inoculation induced none-to-mild diarrhea and lower, delayed fecal viral shedding. Although PEDVPT-P96 elicited slightly lower neutralizing antibodies and PEDV-specific immunoglobulin G (IgG) and immunoglobulin A (IgA) titers, a reduction in pathogenicity and viral shedding of the subsequent challenge with PEDVPT-P5 were noted in both PEDVPT-P5-and PEDVPT-P96-inoculated pigs. Alignment and comparison of full-length sequences of PEDVPT-P5 and PEDVPT-P96 revealed 23 nucleotide changes and resultant 19 amino acid substitutions in non-structure proteins 2, 3, 4, 9, 14, 15, spike, open reading frame 3 (ORF3), and membrane proteins with no detectable deletion or insertion. The present study confirmed the pathogenicity of the PEDVPT isolate in conventional post-weaning pigs. Moreover, data regarding viral attenuation and potency of induced antibodies against PEDVPT-P5 identified PEDVPT-P96 as a potential live-attenuated vaccine candidate.

引用

页数：15

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