Affinity purification of specific chromatin segments from chromosomal loci in yeast

被引：38

作者：

Griesenbeck, J ^{[1
]}

Boeger, H ^{[1
]}

Strattan, JS ^{[1
]}

Kornberg, RD ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 2003年 / 23卷 / 24期

关键词：

D O I：

10.1128/MCB.23.24.9275-9282.2003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-copy gene and promoter regions have been excised from yeast chromosomes and have been purified as chromatin by conventional and affinity methods. Promoter regions isolated in transcriptionally repressed and activated states maintain their characteristic chromatin structures. Gel filtration analysis establishes the uniformity of the transcriptionally activated state. Activator proteins interact in the manner anticipated from previous studies in vivo. This work opens the way to the direct study of specific gene regions of eukaryotic chromosomes in diverse functional and structural states.

引用

页码：9275 / 9282

页数：8

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