The Effects of Zearalenone on the Localization and Expression of Reproductive Hormones in the Ovaries of Weaned Gilts

被引:17
作者
Wan, Boyang [1 ]
Yuan, Xuejun [2 ]
Yang, Weiren [1 ]
Jiao, Ning [1 ]
Li, Yang [1 ]
Liu, Faxiao [1 ]
Liu, Mei [1 ]
Yang, Zaibin [1 ]
Huang, Libo [1 ]
Jiang, Shuzhen [1 ]
机构
[1] Shandong Agr Univ, Dept Anim Sci & Technol, 61 Daizong St, Tai An 271018, Shandong, Peoples R China
[2] Shandong Agr Univ, Dept Life Sci, 61 Daizong St, Tai An 271018, Shandong, Peoples R China
关键词
zearalenone; gilts; ovary; hormone; FOLLICULAR DEVELOPMENT; GROWTH-PERFORMANCE; PRECOCIOUS PUBERTY; FEMALE RATS; GNRH SURGE; EXPOSURE; RECEPTOR; ESTRADIOL; FSHR; MONTMORILLONITE;
D O I
10.3390/toxins13090626
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
This study aims to investigate the effects of zearalenone (ZEA) on the localizations and expressions of follicle stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), gonadotropin releasing hormone (GnRH) and gonadotropin releasing hormone receptor (GnRHR) in the ovaries of weaned gilts. Twenty 42-day-old weaned gilts were randomly allocated into two groups, and treated with a control diet and a ZEA-contaminated diet (ZEA 1.04 mg/kg), respectively. After 7-day adjustment, gilts were fed individually for 35 days and euthanized for blood and ovarian samples collection before morning feeding on the 36th day. Serum hormones of E-2, PRG, FSH, LH and GnRH were determined using radioimmunoassay kits. The ovaries were collected for relative mRNA and protein expression, and immunohistochemical analysis of FSHR, LHR, GnRH and GnRHR. The results revealed that ZEA exposure significantly increased the final vulva area (p < 0.05), significantly elevated the serum concentrations of estradiol, follicle stimulating hormone and GnRH (p < 0.05), and markedly up-regulated the mRNA and protein expressions of FSHR, LHR, GnRH and GnRHR (p < 0.05). Besides, the results of immunohistochemistry showed that the immunoreactive substances of ovarian FSHR, LHR, GnRH and GnRHR in the gilts fed the ZEA-contaminated diet were stronger than the gilts fed the control diet. Our findings indicated that dietary ZEA (1.04 mg/kg) could cause follicular proliferation by interfering with the localization and expression of FSHR, LHR, GnRH and GnRHR, and then affect the follicular development of weaned gilts.
引用
收藏
页数:14
相关论文
共 50 条
  • [41] Ameliorative effects of Bacillus subtilis ANSB01G on zearalenone toxicosis in pre-pubertal female gilts
    Zhao, Lihong
    Lei, Yuanpei
    Bao, Yinghui
    Jia, Ru
    Ma, Qiugang
    Zhang, Jianyun
    Chen, Jun
    Ji, Cheng
    FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2015, 32 (04): : 617 - 625
  • [42] Protective effects of dietary supplementation of Bacillus Subtilis MZ18 against the reproductive toxicity of zearalenone in pregnant rats
    Shi, Zhuo
    Wu, Zhibo
    Wang, Zhongyu
    Liu, Tianshu
    Xie, Ting
    Liu, Nengwen
    Li, Feng
    Yan, Junshu
    ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2025, 292
  • [43] Effects of birthweight on reproductive system development and onset of puberty in gilts
    Almeida, F. R. C. L.
    Laurenssen, B.
    Pereira, L. X.
    Teerds, K. J.
    Soede, N. M.
    REPRODUCTION FERTILITY AND DEVELOPMENT, 2017, 29 (02) : 254 - 261
  • [44] Effects of zearalenone on the localization and expression of the growth hormone receptor gene in the uteri of post-weaning piglets
    Zhou, Min
    Yang, Li Jie
    Yang, Wei Ren
    Huang, Li Bo
    Zhou, Xue Mei
    Jiang, Shu Zhen
    Yang, Zai Bin
    ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 2018, 31 (01): : 32 - 39
  • [45] EFFECTS OF NEONATAL ADMINISTRATION OF ZEARALENONE ON THE REPRODUCTIVE PHYSIOLOGY OF FEMALE MICE
    ITO, Y
    OHTSUBO, K
    JOURNAL OF VETERINARY MEDICAL SCIENCE, 1994, 56 (06) : 1155 - 1159
  • [46] Effects of a ground raw soybean diet on reproductive performance in gilts
    Sykes, D.
    Necaise, K.
    Brookshire, W.
    Gerard, P.
    Cunningham, F.
    Crenshaw, M.
    Ryan, P.
    JOURNAL OF DAIRY SCIENCE, 2007, 90 : 619 - 619
  • [47] Influence of deoxynivalenol and zearalenone on the immunohistochemical expression of oestrogen receptors and liver enzyme genes in vivo in prepubertal gilts
    Magdalena Gajęcka
    Iwona Otrocka-Domagała
    Paweł Brzuzan
    Łukasz Zielonka
    Michał Dąbrowski
    Maciej T. Gajęcki
    Archives of Toxicology, 2023, 97 : 2155 - 2168
  • [48] Effect of zearalenone on reproductive parameters and expression of selected testicular genes in mice
    Zatecka, E.
    Ded, L.
    Elzeinova, F.
    Kubatova, A.
    Dorosh, A.
    Margaryan, H.
    Dostalova, P.
    Korenkova, V.
    Hoskova, K.
    Peknicova, J.
    REPRODUCTIVE TOXICOLOGY, 2014, 45 : 20 - 30
  • [49] Effects of a ground raw soybean diet on reproductive performance in gilts
    Sykes, D.
    Necaise, K.
    Brookshire, W.
    Gerard, P.
    Cunningham, F.
    Crenshaw, M.
    Ryan, P.
    JOURNAL OF ANIMAL SCIENCE, 2007, 85 : 619 - 619
  • [50] Effect of Immunocastration and Diet on Growth Performance, Serum Metabolites and Sex Hormones, Reproductive Organ Development and Carcass Quality of Heavy Gilts
    Perez-Ciria, Leticia
    Javier Miana-Mena, Francisco
    Victoria Falceto, Maria
    Mitjana, Olga
    Angeles Latorre, Maria
    ANIMALS, 2021, 11 (07):