Determining the Composition of Active Cholesterol in the Plasma Membrane of Single Cells by using Electrochemiluminescence

被引：8

作者：

Zuo, Huanzhen ^{[1
,3
]}

Wang, Rui ^{[1
]}

Jiang, Dechen ^{[2
]}

Fang, Danjun ^{[1
]}

机构：

[1] Nanjing Med Univ, Sch Pharm, Nanjing 210029, Jiangsu, Peoples R China

[2] Nanjing Univ, Sch Chem & Chem Engn, Nanjing 210092, Jiangsu, Peoples R China

[3] NanJing Biopharmaceut Innovat Platform Co Ltd, Nanjing 210000, Jiangsu, Peoples R China

来源：

CHEMELECTROCHEM | 2017年 / 4卷 / 07期

基金：

中国国家自然科学基金;

关键词：

active cholesterol; cholesterol composition; electrochemiluminescence; luminol; single cells; LIPID INTERACTIONS; DEHYDROERGOSTEROL;

D O I：

10.1002/celc.201600738

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

Active and inactive cholesterol in the plasma membrane of one cell were co-measured by using luminol electrochemiluminescence, so that the composition of active membrane cholesterol was determined at single cells. The cellular plasma membrane remained intact after the removal of active membrane cholesterol and, thus, inactive cholesterol was activated for reaction with cholesterol oxidase. By utilizing the reactions of active and inactive cholesterol with oxidase in series, both of them generated hydrogen peroxide, inducing the corresponding electrochemiluminescence for the respective quantitation of these two states of membrane cholesterol. The successful determination of active and inactive membrane cholesterol at one cell provides information about the composition of active membrane cholesterol, which is significant for the study of cellular cholesterol trafficking.

引用

页码：1677 / 1680

页数：4

共 19 条

[1] Assess the nature of cholesterol-lipid interactions through the chemical potential of cholesterol in phosphatidylcholine bilayers [J].

Ali, Md Rejwan ;

Cheng, Kwan Hon ;

Huang, Juyang .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (13) :5372-5377

[2] Multichannel electrochemiluminescence of luminol in neutral and alkaline aqueous solutions on a gold nanoparticle self-assembled electrode [J].

Cui, H ;

Xu, Y ;

Zhang, ZF .

ANALYTICAL CHEMISTRY, 2004, 76 (14) :4002-4010

[3] A microscopic interaction model of maximum solubility of cholesterol in lipid bilayers [J].

Huang, JY ;

Feigenson, GW .

BIOPHYSICAL JOURNAL, 1999, 76 (04) :2142-2157

[4] Cellular cholesterol trafficking and compartmentalization [J].

Ikonen, Elina .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2008, 9 (02) :125-138

[5] CELLULAR CHOLESTEROL EFFLUX MEDIATED BY CYCLODEXTRINS [J].

KILSDONK, EPC ;

YANCEY, PG ;

STOUDT, GW ;

BANGERTER, FW ;

JOHNSON, WJ ;

PHILLIPS, MC ;

ROTHBLAT, GH .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (29) :17250-17256

[6]

LANGE Y, 1992, J LIPID RES, V33, P315

[7] How cholesterol homeostasis is regulated by plasma membrane cholesterol in excess of phospholipids [J].

Lange, Y ;

Ye, J ;

Steck, TL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (32) :11664-11667

[8] CHOLESTEROL OXIDASE SUSCEPTIBILITY OF THE RED-CELL MEMBRANE [J].

LANGE, Y ;

MATTHIES, H ;

STECK, TL .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 769 (03) :551-562

[9] Intracellular cholesterol transport [J].

Liscum, L ;

Munn, NJ .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1999, 1438 (01) :19-37

[10] Cholesterol and ergosterol superlattices in three-component liquid crystalline lipid bilayers as revealed by dehydroergosterol fluorescence [J].

Liu, F ;

Sugar, IP ;

Chong, PLG .

BIOPHYSICAL JOURNAL, 1997, 72 (05) :2243-2254

← 1 2 →