Quantitative second-harmonic generation microscopy for imaging porcine cortical bone: Comparison to SEM and its potential to investigate age-related changes

被引:57
作者
Ambekar, Raghu [2 ]
Chittenden, Michael
Jasiuk, Iwona [3 ]
Toussaint, Kimani C., Jr. [1 ,2 ,3 ]
机构
[1] Univ Illinois, Dept Mech Sci & Engn, Mech Engn Lab 2119, Urbana, IL 61801 USA
[2] Univ Illinois, Dept Elect & Comp Engn, Urbana, IL 61801 USA
[3] Univ Illinois, Dept Bioengn, Urbana, IL 61801 USA
基金
美国国家科学基金会;
关键词
Second-harmonic generation microscopy; Scanning electron microscopy; Image analysis; Bone development; Collagen type I; COLLAGEN FIBER ORIENTATION; IN-VIVO; COMPACT-BONE; OSTEOCYTE; CELLS; SITE;
D O I
10.1016/j.bone.2011.11.013
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We propose the use of second-harmonic generation (SHG) microscopy for imaging collagen fibers in porcine femoral cortical bone. The technique is compared with scanning electron microscopy (SEM). SHG microscopy is shown to have excellent potential for hone imaging primarily due its intrinsic specificity to collagen fibers, which results in high contrast images without the need for specimen staining. Furthermore, this technique's ability to quantitatively assess collagen fiber organization is evaluated through an exploratory examination of bone structure as a function of age, from very young to mature bone. In particular, four different age groups: 1 month, 3.5 months, 6 months, and 30 months, were studied. Specifically, we employ the recently developed Fourier transform-second harmonic generation (FT-SHG) imaging technique for the quantification of the structural changes, and observe that as the bone develops, there is an overall reduction in porosity, the number of osteons increases, and the collagen fibers become comparatively more organized. It is also observed that the variations in structure across the whole cross-section of the bone increase with age. The results of this work show that quantitative SHG microscopy can serve as a valuable tool for evaluating the structural organization of collagen fibers in ex vivo bone studies. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码:643 / 650
页数:8
相关论文
共 47 条
[31]  
Ross M.H., 2010, HISTOLOGY TEXT ATLAS, V5th
[32]   Mechanisms of disease - Bone quality - The material and structural basis of bone strength and fragility [J].
Seeman, Ego ;
Delmas, Pierre D. .
NEW ENGLAND JOURNAL OF MEDICINE, 2006, 354 (21) :2250-2261
[33]   Quantitative analysis of collagen fiber organization in injured tendons using Fourier transform-second harmonic generation imaging [J].
Sivaguru, Mayandi ;
Durgam, Sushmitha ;
Ambekar, Raghu ;
Luedtke, David ;
Fried, Glenn ;
Stewart, Allison ;
Toussaint, Kimani C., Jr. .
OPTICS EXPRESS, 2010, 18 (24) :24983-24993
[34]   Does the degree of laminarity correlate with site-specific differences in collagen fibre orientation in primary bone? An evaluation in the turkey ulna diaphysis [J].
Skedros, JG ;
Hunt, KJ .
JOURNAL OF ANATOMY, 2004, 205 (02) :121-134
[35]   Are uniform regional safety factors an objective of adaptive modeling/remodeling in cortical bone? [J].
Skedros, JG ;
Dayton, MR ;
Sybrowsky, CL ;
Bloebaum, RD ;
Bachus, KN .
JOURNAL OF EXPERIMENTAL BIOLOGY, 2003, 206 (14) :2431-2439
[36]   Ontogenetic and regional morphologic variations in the turkey ulna diaphysis: Implications for functional adaptation of cortical bone [J].
Skedros, JG ;
Hunt, KJ ;
Hughes, PE ;
Winet, H .
ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY, 2003, 273A (01) :609-629
[37]   Second harmonic imaging and scoring of collagen in fibrotic tissues [J].
Strupler, M. ;
Pena, A. -M. ;
Hernest, M. ;
Tharaux, P. -L ;
Martin, J. -L. ;
Beaurepaire, E. ;
Schanne-Klein, M. -C. .
OPTICS EXPRESS, 2007, 15 (07) :4054-4065
[38]  
Su SC, 1999, J EXP BIOL, V202, P3239
[39]   The role of collagen in bone structure: An image processing approach [J].
Tzaphlidou, M .
MICRON, 2005, 36 (7-8) :593-601
[40]   Collagen fibril diameter in relation to bone site. A quantitative ultrastructural study [J].
Tzaphlidou, M ;
Berillis, P .
MICRON, 2005, 36 (7-8) :703-705