Formation and transmission of Staphylococcus aureus (including MRSA) aerosols carrying antibiotic-resistant genes in a poultry farming environment

被引：40

作者：

Liu, Dunjiang ^{[1
]}

Chai, Tongjie ^{[1
]}

Xia, Xianzhu ^{[2
]}

Gao, Yuwei ^{[2
]}

Cai, Yumei ^{[1
]}

Li, Xiaoxia ^{[3
]}

Miao, Zengming ^{[3
]}

Sun, Lingyu ^{[1
]}

Hao, Haiyu ^{[1
]}

Roesler, Uwe ^{[4
]}

Wang, Jian

机构：

[1] Shandong Agr Univ, Coll Anim Sci & Vet Med, Sinogerman Cooperat Res Ctr Zoonosis Anim Origin, Tai An 271018, Shandong, Peoples R China

[2] Inst Mil Vet PLA, Changchun, Peoples R China

[3] Taishan Med Univ, Dept Pathogen Biol, Tai An, Shandong, Peoples R China

[4] Free Univ Berlin, Inst Anim Hyg & Environm Hlth, Berlin, Germany

来源：

SCIENCE OF THE TOTAL ENVIRONMENT | 2012年 / 426卷

关键词：

Chicken farming environment; Aerosols of Staphylococcus aureus including MRSA; REP-PCR tracing; Antibiotic-resistance; Antibiotic-resistant genes; Significance to epidemiology and public health; AIRBORNE BACTERIA; STRAINS; PCR; IDENTIFICATION; PREVALENCE; EVOLUTION;

D O I：

10.1016/j.scitotenv.2012.03.060

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

There is a rather limited understanding concerning the antibiotic-resistance of the airborne S. aureus and the transmission of the antibiotic-resistant genes it carries Therefore, we isolated 149 S. aureus strains from the samples collected from the feces, the indoor air and the outdoor air of 6 chicken farms, and performed the research on them with 15 types of antibiotics and the REP-PCR trace identification. The 100% homologous strains were selected to conduct the research on the carrying and transmission status of the antibiotic-resistant genes. The results revealed that 5.37% strains (8/149) were resistant to methicillins (MRSA), and 94% strains (140/149) were resistant to compound sulfamethoxazole, etc. In addition, these strains displayed a resistance to multiple antibiotics (4, 5 or 6 types) and there were also 3 strains resistant to 9 antibiotics. It should be noted that the antibiotic-resistance of some strains isolated from the feces, the indoor and outdoor air was basically the same, and the strains with the same REP-PCR trace identification result carried the same type of antibiotic-resistant genes. The results showed that airborne transmission not only causes the spread of epidemic diseases but also exerts threats to the public health of a community. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：139 / 145

页数：7

共 31 条

[1] NEW SAMPLER FOR THE COLLECTION, SIZING, AND ENUMERATION OF VIABLE AIRBORNE PARTICLES [J].

ANDERSEN, AA .

JOURNAL OF BACTERIOLOGY, 1958, 76 (05) :471-484

[2]

[Anonymous], INTERNET J INFECT DI

[3] STAPHYLOCOCCAL TOXINS IN HUMAN-DISEASE [J].

ARBUTHNOTT, JP ;

COLEMAN, DC ;

DEAZAVEDO, JS .

JOURNAL OF APPLIED BACTERIOLOGY, 1990, 69 :S101-S107

[4] Characterisation and genetic diversity via REP-PCR of Escherichia coli isolates from polluted waters in southern Brazil [J].

Borges, LGD ;

Dalla Vechia, V ;

Corçao, G .

FEMS MICROBIOLOGY ECOLOGY, 2003, 45 (02) :173-180

[5]

CLSI, 2006, M100S15 CLSI

[6]

Davies B., 1994, PRACTITIONER TEACHER

[7] Prevalence of methicillin-resistant Staphylococcus aureus in meat [J].

de Boer, E. ;

Zwartkruis-Nahuis, J. T. M. ;

Wit, B. ;

Huijsdens, X. W. ;

de Neeling, A. J. ;

Bosch, T. ;

van Oosterom, R. A. A. ;

Vila, A. ;

Heuvelink, A. E. .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2009, 134 (1-2) :52-56

[8] The molecular evolution of methicillin-resistant Staphylococcus aureus [J].

Deurenberg, R. H. ;

Vink, C. ;

Kalenic, S. ;

Friedrich, A. W. ;

Bruggeman, C. A. ;

Stobberingh, E. E. .

CLINICAL MICROBIOLOGY AND INFECTION, 2007, 13 (03) :222-235

[9]

Donaldson A., 1999, MICROBIOL TODAY, V26, P18

[10] Transmission identification of Escherichia coli aerosol in chicken houses to their environments using ERIC-PCR [J].

Duan, HuiYong ;

Chai, TongJie ;

Cai, YuMei ;

Zhong, ZhaoBing ;

Yao, MeiLing ;

Zhang, XingXiao .

SCIENCE IN CHINA SERIES C-LIFE SCIENCES, 2008, 51 (02) :164-173

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