Sink/Source Balance of Leaves Influences Amino Acid Pools and Their Associated Metabolic Fluxes in Winter Oilseed Rape (Brassica napus L.)

被引:14
作者
Dellero, Younes [1 ]
Heuillet, Maud [2 ,3 ]
Marnet, Nathalie [4 ,5 ]
Bellvert, Floriant [2 ,3 ]
Millard, Pierre [2 ]
Bouchereau, Alain [1 ,4 ,5 ]
机构
[1] Univ Rennes II, French Natl Res Inst Agr Food & Environm, Inst Genet Environm & Plant Protect, Agrocampus Ouest,Dept Plant Biol & Breeding, Le Rheu 35653, France
[2] Univ Toulouse, French Natl Res Inst Agr Food & Environm, INSA,TBI,Dept Microbiol & Food Chain, French Natl Ctr Sci Res,Dept Plant Biol & Breedin, Toulouse 31400, France
[3] MetaToul MetaboHUB, Natl Infrastruct Metabol & Flux, Toulouse 33140, France
[4] Univ Rennes II, French Natl Res Inst Agr Food & Environm,Dept Pla, Inst Genet Environm & Plant Protect,Agrocampus Ou, Biopolymers Interact Assembliesm,Plateau Profilag, Le Rheu 35653, France
[5] Univ Rennes II, French Natl Res Inst Agr Food & Environm,Dept Tra, Inst Genet Environm & Plant Protect,Agrocampus Ou, Biopolymers Interact Assembliesm,Plateau Profilag, Le Rheu 35653, France
关键词
metabolomics; isotopically non-stationary steady-state metabolic flux analysis; Brassica napus; winter oilseed rape; sink; source balance; senescence; amino acid metabolism; threonine; valine; proline; SMCSO; 2-OXOGLUTARATE DEHYDROGENASE COMPLEX; LEAF SENESCENCE; NITROGEN REMOBILIZATION; ARABIDOPSIS; PLANT; TRANSPORT; RESPIRATION; CATABOLISM; SINK; INHIBITION;
D O I
10.3390/metabo10040150
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Nitrogen remobilization processes from source to sink tissues in plants are determinant for seed yield and their implementation results in a complete reorganization of the primary metabolism during sink/source transition. Here, we decided to characterize the impact of the sink/source balance on amino acid metabolism in the leaves of winter oilseed rape grown at the vegetative stage. We combined a quantitative metabolomics approach with an instationary N-15-labeling experiment by using [N-15](L)-glycine as a metabolic probe on leaf ranks with a gradual increase in their source status. We showed that the acquisition of the source status by leaves was specifically accompanied by a decrease in asparagine, glutamine, proline and S-methyl-l-cysteine sulphoxide contents and an increase in valine and threonine contents. Dynamic analysis of N-15 enrichment and concentration of amino acids revealed gradual changes in the dynamics of amino acid metabolism with respect to the sink/source status of leaf ranks. Notably, nitrogen assimilation into valine, threonine and proline were all decreased in source leaves compared to sink leaves. Overall, our results suggested a reduction in de novo amino acid biosynthesis during sink/source transition at the vegetative stage.
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页数:16
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