Ursolic Acid Loaded PLGA Nanoparticles: in vitro and in vivo Evaluation to Explore Tumor Targeting Ability on B16F10 Melanoma Cell Lines

被引:41
作者
Baishya, Rinku [1 ]
Nayak, Dipak K. [1 ]
Kumar, Deepak [2 ]
Sinha, Samarendu [3 ]
Gupta, Amit [3 ]
Ganguly, Shantanu [3 ]
Debnath, Mita Chatterjee [1 ]
机构
[1] CSIR Indian Inst Chem Biol, Infect Dis & Immunol Div, 4 Raja SC Mullick Rd, Kolkata 700032, India
[2] Natl Inst Pharmaceut Educ & Res, Dept Nat Prod, Kolkata, India
[3] Thakurpukur Canc Ctr & Welf Home Campus, Reg Radiat Med Ctr, Kolkata, India
关键词
B16F10 cell line; biodistribution; PLGA nanoparticle; radiolabeling; tumor; ursolic acid; PULMONARY DELIVERY; ANTICANCER AGENTS; APOPTOSIS; DERIVATIVES; VORICONAZOLE; INHIBITION; ACTIVATION; PEPTIDES; PATHWAY;
D O I
10.1007/s11095-016-1994-1
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Purpose Ursolic acid (UA), a pentacyclic triterpenoid extracted from plants, shows promising inhibitory effect in different tumor bearing cell lines. In the present study we fabricated UA loaded PLGA nanoparticles (UA-NPs) as the drug carrier and thoroughly evaluated in vitro and in vivo the differential tumor targeting effects of UA and UA-NPs in B16F10 melanoma cells. Methods Ursolic acid loaded PLGA nanoparticles were prepared by emulsion solvent evaporation technique and evaluated for particle size, polydispersity, zeta potential and drug release potency. MTT assay as well as flow cytometric and confocal microscopic analyses were done in B16F10 mouse melanoma cell lines. Formulations were labeled with technetium-99m to evaluate the biodistribution and perform scintigraphic imaging studies following intravenous administration in tumor bearing mice model. Results Single emulsification technique produced smooth spherical nanoparticles of small size with relatively narrow size distribution (154 +/- 4.56 nm). On B16F10 cell line, the formulation showed higher cytotoxicity compared to the free drug due to increased in vitro cellular uptake. The formulation was successfully radiolabeled and remained substantially (>90%) stable when incubated (37 degrees C, 6 h) separately in normal saline or freshly collected rat serum or histidine solution. The radiolabeled UA-NPs exhibited slower blood clearance and comparatively high uptake in tumor region as evidenced by biodistribution and scintigraphic studies. Conclusions The in vitro and in vivo studies have proved the tumor targeting potential of UA-NPs in B16F10 melanoma cell lines.
引用
收藏
页码:2691 / 2703
页数:13
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