The Cyclin-Dependent Kinase Inhibitor p27Kip1 is a Positive Regulator of Schwann Cell Differentiation In Vitro

被引：14

作者：

Li, Honghui ^{[1
,2
]}

Yang, Huiguang ^{[1
,2
]}

Liu, Yonghua ^{[1
,2
]}

Huan, Weipeng ^{[1
,2
]}

Zhang, Shuangwei ^{[1
,2
]}

Wu, Gang ^{[1
,2
]}

Lu, Qiuhui ^{[1
,2
]}

Wang, Qiuhong ^{[1
,2
]}

Wang, Youhua ^{[1
,2
]}

机构：

[1] Nantong Univ, Dept Orthopaed, Affiliated Hosp, Nantong 226001, Peoples R China

[2] Nantong Univ, Dept Orthopaed, Coll Med, Nantong 226001, Peoples R China

来源：

JOURNAL OF MOLECULAR NEUROSCIENCE | 2011年 / 45卷 / 02期

基金：

中国国家自然科学基金;

关键词：

p27(Kip1); Schwann cell; Differentiation; Peripheral nerve; CDK INHIBITOR; UBIQUITIN LIGASE; PERIPHERAL-NERVE; MICE LACKING; G(1) PHASE; P27; PROMOTES; PHOSPHORYLATION; MYELINATION; DEGRADATION;

D O I：

10.1007/s12031-011-9518-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Schwann cell precursors differentiating into a myelinating phenotype are critical for peripheral nerve development and regeneration. However, little is known about the underlying molecular mechanisms of Schwann cell differentiation. In the present study, we performed a cyclic adenosine monophosphate-induced Schwann cell differentiation model in vitro. Western blot analysis showed that p27(Kip1) expression was upregulated during the differentiation of Schwann cell, while the inhibition of p27(Kip1) expression by short hairpin RNA-mediated knockdown significantly abolished the expression of promyelinating markers and the alteration of cellular morphology. In addition, immunofluorescence revealed a decrease of p27(Kip1) nuclear staining and a concomitant increase of cytoplasmic staining in differentiated Schwann cells. In summary, our data indicated that p27(Kip1) was a positive regulator of Schwann cell differentiation in vitro.

引用

页码：277 / 283

页数：7

共 35 条

[1]

Arroyo EJ, 1998, J NEUROSCI, V18, P7891

[2] The claw paw mutation reveals a role for Lgi4 in peripheral nerve development [J].